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Nitrite Colorimetric Assay Kit

Cat.No.:E-BC-K070-S
Detection instrument: Spectrophotometer(550 nm)

Detection method: Colorimetric method

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Detection principle

Nitrite can react with chromogenic agent producing light red azo-compound. The content of nitrite can be calculated indirectly by measuring the OD value at 550 nm.

Performance characteristics

Sample type	Serum,plasma,saliva,tissue,cells,cell culture supernatant
Sensitivity	1.36 μmol/L
Detection range	1.36-500 μmol/L
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	60 min
Precision	Average inter-assay CV: 3.300%Average intra-assay CV: 2.600%
Other instruments required	Micropipettor, Vortex mixer, Centrifuge
Other reagents required	Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage	Reagent 6: -20℃, others: 2-8℃
Valid period	12 months

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

SAUDI JOURNAL OF BIOLOGICAL SCIENCES (2022) IF: 4.052
Evaluation of testicular torsion management in Ogbomoso, South-Western Nigeria and surgical detorsion-augmented treatment with phytochemical fractions of Corchorus olitorius leaf in experimental rats

DOI: 10.1016/j.sjbs.2022.103495

Sample: testicular tissue
Scientific Reports (2022) IF: 4.996
Effects of yoga on cardiometabolic risks and fetomaternal outcomes are associated with serum nitric oxide in gestational hypertension: a randomized control trial

DOI: 10.1038/s41598-022-15216-4

PMID: 35821033
Sample: serum
Journal of Pain Research (2022) IF: 3.133
Anti-Nociceptive and Anti-Inflammatory Activities of the Ethyl Acetate Extract of Belamcanda chinensis (L.) Redouté in Raw 264.7 Cells in vitro and Mouse Model in vivo

DOI: 10.2147/JPR.S353357

PMID: 35502404
Sample: RAW264.7 cell
Evidence-based Complementary and Alternative Medicine (2021) IF: 2.63
Zanthoxylum bungeanum Seed Oil Attenuates LPS-Induced BEAS-2B Cell Activation and Inflammation by Inhibiting the TLR4/MyD88/NF-κB Signaling Pathway

DOI: 10.1155/2021/2073296

Sample: BEAS-2B cell
MOLECULAR BIOLOGY REPORTS (2020) IF: 1.402
Honey protects against chronic unpredictable mild stress induced- intestinal barrier disintegration and hepatic inflammation

DOI: 10.1007/s11033-020-05888-4

PMID: 33047241
Sample: Serum
CLINICAL AND EXPERIMENTAL HYPERTENSION (2021) IF: 1.749
Attenuation of baroreflex sensitivity and heart rate variability is linked to reduced levels of nitric oxide in pregnant women having risks of developing gestational hypertension

DOI: 10.1080/10641963.2021.1883053

PMID: 33567918
Sample: Serum
Toxicology Reports (2022)
Effect of silymarin on blood coagulation profile and osmotic fragility in carbon tetrachloride induced hepatotoxicity in male Wistar rats

DOI: 10.1016/j.toxrep.2022.06.005

Sample: plasma

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