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Non-esterified Free Fatty Acids (NEFA/FFA) Colorimetric Assay Kit

Name: Non-esterified Free Fatty Acids (NEFA/FFA) Colorimetric Assay Kit
Brand: Elabscience
SKU: E-BC-K013-S
Price: 180 USD
Availability: InStock
Rating: 5 (2 reviews)

Citations(14)

Cat.No.:E-BC-K013-S
Detection instrument: Spectrophotometer(715 nm)

Detection method: Colorimetric method

Manual MSDS

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100Assays

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Detection principle

Under the condition of weak acidity, non-esterified free fatty acids (NEFA) react with nantokite to form copper soap, which has a specific absorption peak at 715nm. The content of NEFA can be calculated by measuring the OD value at 715 nm.

Performance characteristics

Synonyms	NEFA
Sample type	Serum,animal tissue
Sensitivity	0.05 mmol/L
Detection range	0.05-2.0 mmol/L
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	60 min
Precision	Average inter-assay CV: 6.100%Average intra-assay CV: 2.200%
Other instruments required	Vortex mixer, Micropipettor
Storage	2-8℃
Valid period	12 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.05-2.0 mmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Rat liver tissue homogenate	1-3
Rat heart tissue homogenate	1
Rat kidney tissue homogenate	1
Mouse liver tissue homogenate	1-3
Rat serum	1
Mouse serum	1
Human serum	1

Note: The diluent is Reagent 1.

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

CELL (2023) IF: 66.849
Light modulates glucose metabolism by a retina-hypothalamus-brown adipose tissue axis

DOI: 10.1016/j.cell.2022.12.024

PMID: 36669474
Sample: Serum
Engineering (2022) IF: 12.834
Hydrogen Sulfide Promotes Adipocyte Differentiation, Hyperplasia, and Hypertrophy

DOI: 10.1016/j.eng.2022.09.010

Sample: adipose tissues,preadipocytes
SCIENCE OF THE TOTAL ENVIRONMENT (2022) IF: 10.753
Combined effect of polystyrene microplastics and bisphenol A on the human embryonic stem cells-derived liver organoids: The hepatotoxicity and lipid accumulation

DOI: 10.1016/j.scitotenv.2022.158585

PMID: 36089014
Sample: liver organoids (LOs)
DIABETES (2022) IF: 9.337
PATAS, a First-in-Class Therapeutic Peptide Biologic, Improves Whole-Body Insulin Resistance and Associated Comorbidities In Vivo

DOI: 10.2337/db22-0058

PMID: 35822820
Sample: blood
iScience (2022) IF: 6.107
MiR-203 is an anti-obese miRNA by targeting ASBT

DOI: 10.1016/j.isci.2022.104708

Sample: plasma
Pharmaceutics (2022) IF: 6.525
The Anti-Obesity Potential of Superparamagnetic Iron Oxide Nanoparticles against High-Fat Diet-Induced Obesity in Rats: Possible Involvement of Mitochondrial Biogenesis in the Adipose Tissues

DOI: 10.3390/pharmaceutics14102134

PMID: 36297569
Sample: serum
Frontiers in Pharmacology (2018) IF: 5.811
Astragaloside IV Inhibits Triglyceride Accumulation in Insulin-Resistant HepG2 Cells via AMPK-Induced SREBP-1c Phosphorylation

DOI: 10.3389/fphar.2018.00345

Sample: HepG2 cells
EUROPEAN JOURNAL OF PHARMACOLOGY (2021) IF: 4.432
Fatty acid synthase inhibition ameliorates diabetes induced liver injury in rodent experimental model

DOI: 10.1016/j.ejphar.2021.174078

PMID: 33839087
Sample: Serum
Drug Design Development and Therapy (2020) IF: 3.216
Radix Polygoni Multiflori and Its Main Component Emodin Attenuate Non-Alcoholic Fatty Liver Disease in Zebrafish by Regulation of AMPK Signaling Pathway

DOI: 10.2147/DDDT.S243893

Sample: Tissue homogenate
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY (2020) IF: 3.292
Chronic exposure of bisphenol A impairs carbohydrate and lipid metabolism by altering corresponding enzymatic and metabolic pathways

DOI: 10.1016/j.etap.2020.103387

PMID: 32339907
Sample: Serum
CLINICA CHIMICA ACTA (2019) IF: 2.735
Serum albumin saturation test based on non-esterified fatty acids imbalance for clinical employment

DOI: 10.1016/j.cca.2019.05.003

Sample: serum
SAUDI JOURNAL OF BIOLOGICAL SCIENCES (2020) IF: 2.802
Anti-obesity potential of Moringa olifera seed extract and lycopene on high fat diet induced obesity in male Sprauge Dawely rats

DOI: 10.1016/j.sjbs.2020.06.026

PMID: 32994733
Sample: Serum
MOLECULAR GENETICS AND GENOMICS (2023) IF: 2.98
Regulation of PPARγ/CPT-1 expression ameliorates cochlear hair cell injury by regulating cellular lipid metabolism and oxidative stress

DOI: 10.1007/s00438-023-01993-8

Sample: House Ear Institute-Organ of Corti 1 cells (HEI-OC
TOXICOLOGY AND INDUSTRIAL HEALTH (2020) IF: 1.708
Elevated, sustained, and yet reversible biotoxicity effects of lead on cessation of exposure: Melatonin is a potent therapeutic option:

DOI: 10.1177/0748233720937199

Sample: Plasma

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Reviews/Q&A

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Reviews
Q&A

Verified Customer

l***sSubmitted [ May 29 2019 ]

Application:Biochemical
Description:I used this biochemical kit to detect the content of free fatty acids in the liver of rats, and the experimental results could be obtained quickly according to the operation steps in the kit instructions.The formula notes in the specification are detailed, and the calculated results are clear and accurate.The sample took a little longer to process, and overall, the kit was excellent.

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Verified Customer

M*ySubmitted [ May 29 2019 ]

Application:Biochemical
Description: kit is accuracy and good reliability ,but the operation is a bit complicated and take a long time to extract samples

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M**********dSubmitted [ Aug 12 2022 ]

Asked: How many cells do I need for an assay?

adminSubmitted [ Aug 12 2022 ]

Answered: At least 10^7 cells is required based on the cell types.

D********dSubmitted [ Aug 01 2022 ]

Asked: Can I use microplate reader for the detection?

adminSubmitted [ Aug 01 2022 ]

Answered: If you'd like to measure by microplate reader, I highly recommend E-BC-K013-M which is designed for microplate reader measurement.

adminSubmitted [ Aug 01 2022 ]

Answered: If you'd like to measure by microplate reader, I highly recommend E-BC-K013-M which is designed for microplate reader measurement.

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