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Non-esterified Free Fatty Acids (NEFA) Colorimetric Assay Kit

Cat.No.:E-BC-K014
Detection instrument: Semi-automatic biochemical analyzer (546 nm), automatic biochemical analyzer (546 nm), Microplate re

Detection method: Colorimetric method

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96T

Price: $516

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Detection principle

NEFA and can react with coenzyme A and form acetyl-CoA under the catalysis of acetyl-CoA-synthetase (ACS). Acetyl-CoA can produce H2O2 when catalyzed by acetyl-CoA-oxidase (ACOD). Then H2O2 react with TOOS and 4-amino-antipyrine (4-APP) to generate a colored substrate under the catalysis of peroxidase (POD). The colored substrate has a maximum absorption peak at 546 nm. Measure the OD value at 546 nm and calculate the NEFA content indirectly.

Performance characteristics

Synonyms	NEFA
Sample type	Serum,plasma,tissue homogenate,cells,cell supernatant
Detection range	0.01-3.0 mmol/L
Detection method	Colorimetric method
Assay type	Quantitative
Other reagents required	Normal saline (0.9% NaCl)
Storage	2-8℃
Valid period	12 months

Images

Liver free fatty acid was significantly increased by high-fat-high-cholesterol diet and DUSP22 knock out. (**P<0.01)

Chenxu Ge et al investigate the effect ROS that inhibits DUSP22 expression in fatty livers. Non-esterified Free Fatty Acid (NEFA) in mouse liver was determined using NEFA colorimetric assay kit (E-BC-K014).

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

IMMUNITY (2022) IF: 43.474
Prolonged hypernutrition impairs TREM2-dependent efferocytosis to license chronic liver inflammation and NASH development

DOI: 10.1016/j.immuni.2022.11.013

PMID: 36521495
Sample: serum
Nature Communications (2022) IF: 17.694
Hepatocyte phosphatase DUSP22 mitigates NASH-HCC progression by targeting FAK

DOI: 10.1038/s41467-022-33493-5

PMID: 36209205
Sample: serum
Nature Communications (2022) IF: 14.919
The E3 ubiquitin-protein ligase Trim31 alleviates non-alcoholic fatty liver disease by targeting Rhbdf2 in mouse hepatocytes

DOI: 10.1038/s41467-022-28641-w

PMID: 35217669
Sample: serum
BMC Endocrine Disorders (2022) IF: 3.263
Low-dose spironolactone ameliorates adipose tissue inflammation and apoptosis in letrozole-induced PCOS rat model

DOI: 10.1186/s12902-022-01143-y

PMID: 36071485
Sample: adipose tissue
Animals (2022) IF: 2.752
Effect of TMR Briquettes on Milk Production, Nutrient Digestibility, and Manure Excretions of Dairy Cows in the Dry Zone of Sri Lanka

DOI: 10.3390/ani12070932

PMID: 35405920
Sample: serum

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Reviews/Q&A

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Reviews
Q&A

M*****iSubmitted [ Oct 21 2022 ]

Asked: My instrument does not have 546 nm, how should I detect it?

adminSubmitted [ Oct 21 2022 ]

Answered: Choose the nearest wavelength if the instrument cannot be set to the wavelength required by this kit.

M*****iSubmitted [ Sep 13 2022 ]

Asked: Is the sample of hemolytic serum available?

adminSubmitted [ Sep 13 2022 ]

Answered: Hemolytic sample will affect the result.

S***********aSubmitted [ Jul 21 2022 ]

Asked: Can I request a quotation for this Cat#E-BC-K014

adminSubmitted [ Jul 21 2022 ]

Answered: The price of 96T E-BC-K014 is 516USD per kit.

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