Non-esterified Free Fatty Acids (NEFA) Colorimetric Assay Kit (E-BC-K014)
For research use only.
| Detection Principle | NEFA and can react with coenzyme A and form acetyl-CoA under the catalysis of acetyl-CoA-synthetase (ACS). Acetyl-CoA can produce H2O2 when catalyzed by acetyl-CoA-oxidase (ACOD). Then H2O2 react with TOOS and 4-amino-antipyrine (4-APP) to generate a colored substrate under the catalysis of peroxidase (POD). The colored substrate has a maximum absorption peak at 546 nm. Measure the OD value at 546 nm and calculate the NEFA content indirectly. |
| Synonyms | NEFA |
| Sample Type | Serum,Plasma,Tissue,Cell,Cell supernatant |
| Detection Method | Colorimetric method |
| Detection Instrument | Semi-automatic biochemical analyzer (546 nm), Automatic biochemical analyzer (546 nm), Microplate reader |
| Research Area | Metabolic Diseases , Glycolysis And Lipid Metabolism |
| Other Reagents Required | Normal saline (0.9% NaCl) |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Detection Range | 0.01-3.0 mmol/L |
| Sample Volume | 4 μL |
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