Non-esterified Free Fatty Acids (NEFA) Colorimetric Assay Kit

    • Biochemical Assay Kit-Elabscience
    • Biochemical Assay Kit-Elabscience
    • Biochemical Assay Kit-Elabscience

      Catalog number:E-BC-K014

      • 96T
      - +
      Price: $516
      Can detect 92 samples if without duplication

      Detection method: Colorimetric method

      Detection instrument: Semi-automatic biochemical analyzer (546 nm), automatic biochemical analyzer (546 nm), Microplate re

      Lead Time: 7~10 daysWelcome to order from local distributors.

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      Detection principle

      NEFA and can react with coenzyme A and form acetyl-CoA under the catalysis of acetyl-CoA-synthetase (ACS). Acetyl-CoA can produce H2O2 when catalyzed by acetyl-CoA-oxidase (ACOD). Then H2O2 react with TOOS and 4-amino-antipyrine (4-APP) to generate a colored substrate under the catalysis of peroxidase (POD). The colored substrate has a maximum absorption peak at 546 nm. Measure the OD value at 546 nm and calculate the NEFA content indirectly.

      Performance characteristics

      Synonyms NEFA
      Sample type serum,plasma,tissue homogenate,cells,cell supernatant
      Detection range 0.01-3.0 mmol/L
      Other reagents required Normal saline (0.9% NaCl)
      Storage 2-8℃
      Valid period 6 months
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      MSDS for ELISA ISO 9001

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