Non-esterified Free Fatty Acids (NEFA) Colorimetric Assay Kit


      Catalog number:E-BC-K013-S

      • 100Assays
      - +
      Price: $180
      Can detect 50 samples if without duplication

      Detection method: Colorimetric method

      Detection instrument: Spectrophotometer(715 nm)

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      Detection principle

      Under the condition of weak acidity, non-esterified free fatty acids (NEFA) react with nantokite to form copper soap, which has a specific absorption peak at 715nm. The content of NEFA can be calculated by measuring the OD value at 715 nm.

      Performance characteristics

      Synonyms NEFA
      Sample type Serum,animal tissue,cells
      Sensitivity 0.05 mmol/L
      Detection range 0.05-2.0 mmol/L
      Detection method Colorimetric method
      Assay type Quantitative
      Assay time 60 min
      Precision Average inter-assay CV: 6.1%Average intra-assay CV: 2.2%
      Other instruments required Vortex mixer, Micropipettor
      Storage 2-8℃
      Valid period 6 months

      Dilution of sample

      It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.05-2.0 mmol/L).

      The recommended dilution factor for different samples is as follows (for reference only):

      Sample type

      Dilution factor

       Rat liver tissue homogenate


       Rat heart tissue homogenate


               Rat kidney tissue homogenate


       Mouse liver tissue homogenate


      Rat serum


      Mouse serum


      Human serum


      Note: The diluent is Reagent 1.


      1. Radix Polygoni Multiflori and Its Main Component Emodin Attenuate Non-Alcoholic Fatty Liver Disease in Zebrafish by Regulation of AMPK Signaling Pathway
        IF: 3.208
        Journal: Drug Design, Development and Therapy(2020)
        DOI: 10.2147/DDDT.S243893
        PMID: 99999999
        Products: E-BC-K013-S 
        Sample type: Tissue homogenate
        Reactivity: Fish
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      • Reviews (2)
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      Verified Customer

      l***sSubmitted [ May 29 2019 ]

      • Application:Biochemical
      • Description:I used this biochemical kit to detect the content of free fatty acids in the liver of rats, and the experimental results could be obtained quickly according to the operation steps in the kit instructions.The formula notes in the specification are detailed, and the calculated results are clear and accurate.The sample took a little longer to process, and overall, the kit was excellent.
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      Verified Customer

      M*ySubmitted [ May 29 2019 ]

      • Application:Biochemical
      • Description: kit is accuracy and good reliability ,but the operation is a bit complicated and take a long time to extract samples
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