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Non-esterified Free Fatty Acids (NEFA) Colorimetric Assay Kit

Citations(1)Uniprot : zzdx
  • Cat.No.:E-BC-K013-S

  • Detection instrument: Spectrophotometer(715 nm)

To Purchase E-BC-K013-S

  • 100Assays
Price: $180

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Detection principle

Under the condition of weak acidity, non-esterified free fatty acids (NEFA) react with nantokite to form copper soap, which has a specific absorption peak at 715nm. The content of NEFA can be calculated by measuring the OD value at 715 nm.

Performance characteristics

Synonyms NEFA
Sample type Serum,animal tissue,cells
Sensitivity 0.05 mmol/L
Detection range 0.05-2.0 mmol/L
Detection method Colorimetric method
Assay type Quantitative
Assay time 60 min
Precision Average inter-assay CV: 6.1%Average intra-assay CV: 2.2%
Other instruments required Vortex mixer, Micropipettor
Storage 2-8℃
Valid period 6 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.05-2.0 mmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

 Rat liver tissue homogenate


 Rat heart tissue homogenate


         Rat kidney tissue homogenate


 Mouse liver tissue homogenate


Rat serum


Mouse serum


Human serum


Note: The diluent is Reagent 1.


From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Drug Design, Development and Therapy (2020) IF: 3.208
    Radix Polygoni Multiflori and Its Main Component Emodin Attenuate Non-Alcoholic Fatty Liver Disease in Zebrafish by Regulation of AMPK Signaling Pathway

    DOI: 10.2147/DDDT.S243893

    PMID: 99999999

    Sample: Tissue homogenate


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Verified Customer

l***sSubmitted [ May 29 2019 ]

  • Application:Biochemical
  • Description:I used this biochemical kit to detect the content of free fatty acids in the liver of rats, and the experimental results could be obtained quickly according to the operation steps in the kit instructions.The formula notes in the specification are detailed, and the calculated results are clear and accurate.The sample took a little longer to process, and overall, the kit was excellent.
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Verified Customer

M*ySubmitted [ May 29 2019 ]

  • Application:Biochemical
  • Description: kit is accuracy and good reliability ,but the operation is a bit complicated and take a long time to extract samples
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