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One-component TMB Substrate

  • Cat.No.:E-IR-R201

  • Application: ELISA

To Purchase E-IR-R201

Size:
  • 30 mL
  • 100 mL
  • 500 mL
  • 1000 mL
Price: $33
Qty:

Introduction

TMB is a popular chromogenic substrate for HRP detection in ELISA and is available in several formats. The soluble One-component TMB Substrate are supplied as Ready-to-Use mono-component liquids that require no preparation before use. The product mainly contains 3,3’,5,5’-tetramethylbenzidine and contains no DMF or DMSO.
One-component TMB Substrate yields a blue color (Amax= 370 nm and 652 nm) when reacted with peroxidase in microwell applications. The product changes to yellow (Amax = 450 nm) upon addition of a sulfuric or phosphoric acid stop solution.

Method for use

1. Add 100 μL of the TMB Substrate Solution to each microplate well.
2. Incubate for 5~30 min according to the reaction system.
3. Stop reaction by adding 50 uL stop solution (1M HCl or 1M H2SO4).
4. Measure the absorbance of each well at 450 nm.

Storage

2-8℃, 12 months
Shading light.

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Nanomaterials (2022) IF: 5.719
    Surface-Enhanced Carboxyphenyl Diazonium Functionalized Screen-Printed Carbon Electrode for the Screening of Tuberculosis in Sputum Samples

    DOI: 10.3390/nano12152551

    PMID: 35893519

  2. Vaccines (2021) IF: 4.422
    K-Ras Peptide Mimotope Induces Antigen Specific Th1 and B-Cell Immune Responses against G12A-Mutated K-Ras Antigen in Balb/c Mice

    DOI: 10.3390/vaccines9030195

    PMID: 33652552

    Sample: intra and extra-cellular total protein samples fro
  3. Tropical Medicine and Infectious Disease (2022) IF: 3.711
    Non-Invasive Detection of SARS-CoV-2 Antigen in Saliva versus Nasopharyngeal Swabs Using Nanobodies Conjugated Gold Nanoparticles

    DOI: 10.3390/tropicalmed7060102

    PMID: 35736981

  4. PeerJ (2018) IF: 2.118
    In silico-guided sequence modifications of K-ras epitopes improve immunological outcome against G12V and G13D mutant KRAS antigens

    DOI: 10.7717/peerj.5056

    Sample: Serum
  5. BIOSCIENCE REPORTS (2019) IF: 2.535
    Tumor suppressor properties of the small C-terminal domain phosphatases in non-small cell lung cancer

    DOI: 10.1042/BSR20193094

    PMID: 31774910

    Sample: Cell lysate
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