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When cells undergo apoptosis, specific DNA endonucleases will be activated, cutting the genomic DNA between the nucleosomes. The DNA of apoptotic cells is cleaved into multimers of 180~200bp fragments, corresponding to the oligonucleosomal size. Therefore, the DNA of apoptotic cells typically migrates as a ladder of 180~200bp on an agarose gel. The exposed 3'-OH of the broken DNA can be catalyzed by Terminal Deoxynucleotidyl Transferase (TdT) with fluorescein labeled dUTP, which can be detected with flow cytometry.
|Detection method||Fluorometric Method|
|Sample type||Cell samples|
|Assay time||2.5-3 hours|
|Detection instrument||Flow Cytometry|
|Dye Type||Elab Fluor® Violet 450|
|Channel set||Pacific Blue|
|Other reagents required||PBS buffer (with 1%BSA) (PH7.2 ~ 7.4)|
|Storage||-20°C, shading light|
|Expiration date||12 months|
|E-CK-A233||Annexin V-Elab Fluor® Violet 450/PI Apoptosis Kit|
|E-CK-A301||Mitochondrial Membrane Potential Assay Kit (with JC-1)|
|E-CK-A362||Enhanced Cell Counting Kit 8 (WST-8/CCK8)|
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