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One-step TUNEL Flow Cytometry Apoptosis Kit (Green, FITC)

  • Cat.No.:E-CK-A420

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Price: $198

Detection principle

When cells undergo apoptosis, specific DNA endonucleases will be activated, cutting the genomic DNA between the nucleosomes. The DNA of apoptotic cells is cleaved into multimers of 180~200bp fragments, corresponding to the oligonucleosomal size. Therefore, the DNA of apoptotic cells typically migrates as a ladder of 180~200bp on an agarose gel. The exposed 3'-OH of the broken DNA can be catalyzed by Terminal Deoxynucleotidyl Transferase (TdT) with fluorescein labeled dUTP, which can be detected with flow cytometry.

General Information

Application DNA Fragmentation
Detection method Fluorometric Method
Sample type Cell samples
Assay time 2.5-3 hours
Detection instrument Flow Cytometry
Dye Type FITC
Ex/Em (nm) 490/530
Channel set FITC
Other reagents required PBS buffer (with 1%BSA) (PH7.2 ~ 7.4)
Storage -20°C, shading light
Shipping Ice bag
Expiration date 12 months



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