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One-step TUNEL In Situ Apoptosis Kit (Green, FITC) (E-CK-A320)

  • +5
All Size Price Qty
100Assays $ 498.00
50Assays $ 298.00
20Assays $ 198.00
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For research use only.

Detection Principle When cells undergo apoptosis, specific DNA endonucleases will be activated, cutting the genomic DNA between the nucleosomes. The DNA of apoptotic cells is cleaved into multimers of 180~200bp fragments, corresponding to the oligonucleosomal size. Therefore, the DNA of apoptotic cells typically migrates as a ladder of 180~200bp on an agarose gel. The exposed 3'-OH of the broken DNA can be catalyzed by Terminal Deoxynucleotidyl Transferase (TdT) with fluorescein labeled dUTP, which can be detected with fluorescence microscope.
Detection Method Fluorometric method
Sample Type Paraffin section;frozen section;cell slide
Assay Time 3 hours
Detection Instrument Fluorescence Microscope
Dye Type FITC
Ex/Em 490/520
Channel Set FITC
Filter Set FITC
Other Reagents Required 4% Paraformaldehyde(E-IR-R113),0.2% Triton X-100(E-IR-R122),PBS(E-BC-R187),ddH2O,Mounting solution with anti-fluorescence quencher(E-IR-R119),Xylene,Ethanol
Storage This product can be stored at -20°C for 12 months with shading light.
Expiration date 12 months
Shipping Ice bag
Cat.No. Product Name Sizes
E-CK-A211 Annexin V-FITC/PI Apoptosis Kit 200Assays , 100Assays , 50Assays , 20Assays
E-CK-A301 Mitochondrial Membrane Potential Assay Kit (with JC-1) 100Assays , 50Assays , 20Assays
E-CK-A321 One-step TUNEL In Situ Apoptosis Kit (Green, Elab Fluor® 488) 100Assays , 50Assays , 20Assays
E-CK-A362 Enhanced Cell Counting Kit 8 (WST-8/CCK8) 500Tests , 100Tests
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