Click here to view cell apoptosis assay products

One-step TUNEL In Situ Apoptosis Kit (Red, Elab Fluor® 647)

 Click to find the fluorescence fit you.

  • Cat.No.:E-CK-A324

To Purchase E-CK-A324

  • 20Assays
  • 50Assays
  • 100Assays
Price: $198

Detection principle

When cells undergo apoptosis, specific DNA endonucleases will be activated, cutting the genomic DNA between the nucleosomes. The DNA of apoptotic cells is cleaved into multimers of 180~200bp fragments, corresponding to the oligonucleosomal size. Therefore, the DNA of apoptotic cells typically migrates as a ladder of 180~200bp on an agarose gel. The exposed 3'-OH of the broken DNA can be catalyzed by Terminal Deoxynucleotidyl Transferase (TdT) with fluorescein labeled dUTP, which can be detected with fluorescence microscope.

General Information

Application DNA Fragmentation
Detection method Fluorometric Method
Sample type Paraffin section, frozen section, cell slide
Assay time 3 hours
Detection instrument Fluorescence Microscope
Dye Type Elab Fluor® 647
Ex/Em (nm) 650/665
Filter set Cy5
Other reagents required 4% Paraformaldehyde(E-IR-R113),0.2% Triton X-100(E-IR-R122),PBS(E-IR-R187),ddH2O,Mounting solution with anti-fluorescence quencher(E-IR-R119),Xylene,Ethanol
Storage -20°C, shading light
Shipping Ice bag
Expiration date 12 months

Operation Guide


    Knockdown of UAF1 alleviates sevoflurane-induced cognitive impairment and neurotoxicity in rats by inhibiting pro-inflammatory signaling and oxidative stress

    DOI: 10.2131/jts.47.349

    PMID: 36047109

  2. Biopreservation and Biobanking (2021) IF: 2.3
    Effects of AavLEA1 Protein on Mouse Ovarian Tissue Cryopreservation by Vitrification

    DOI: 10.1089/bio.2021.0048

    PMID: 34788107

  3. Journal of Stroke & Cerebrovascular Diseases (2023) IF: 2.677
    Inhibition of PI3K/Akt/mTOR signaling by NDRG2 contributes to neuronal apoptosis and autophagy in ischemic stroke

    DOI: 10.1016/j.jstrokecerebrovasdis.2023.106984

  4. RENAL FAILURE (2022) IF: 3.222
    Rosiglitazone attenuates hypoxia-induced renal cell apoptosis by inhibiting NF-κB signaling pathway in a PPARγ-dependent manner

    DOI: 10.1080/0886022X.2022.2148539

  5. Journal of Immunology Research (2022) IF: 4.818
    miR-214 Modulates the Growth and Migration of Oral Cancer before and after Chemotherapy through Mediating ULK1

    DOI: 10.1155/2022/4589182

    PMID: 35692501

  6. DRUG DELIVERY (2022) IF: 6.419
    Sevelamer arsenite nanoparticle as a Pi-responsive drug carrier and embolic agent for chemoembolization

    DOI: 10.1080/10717544.2022.2072541

  7. Nature Communications (2022) IF: 17.694
    Long-term monitoring of intravital biological processes using fluorescent protein-assisted NIR-II imaging

    DOI: 10.1038/s41467-022-34274-w

    PMID: 36333308

Show AllShorten


  • Show all
  • Reviews
  • Q&A

N*** Submitted [ May 04 2024 ]

Asked: can I perform this assay in a drosophila melanogaster ( fruit fly) sample?



adminSubmitted [ May 04 2024 ]

Answered: Hello Neha,we will reply you via E-mail later.

... Show All Show Less

People Also Bought

Apply for
*Product Name:
*Catalog Number:
*When will you use it?