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One-step TUNEL In Situ Apoptosis Kit (Red, Elab Fluor® 555)

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  • Cat.No.:E-CK-A325

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Price: $198

Detection principle

When cells undergo apoptosis, specific DNA endonucleases will be activated, cutting the genomic DNA between the nucleosomes. The DNA of apoptotic cells is cleaved into multimers of 180~200bp fragments, corresponding to the oligonucleosomal size. Therefore, the DNA of apoptotic cells typically migrates as a ladder of 180~200bp on an agarose gel. The exposed 3'-OH of the broken DNA can be catalyzed by Terminal Deoxynucleotidyl Transferase (TdT) with fluorescein labeled dUTP, which can be detected with fluorescence microscope.

General Information

Application DNA Fragmentation
Detection method Fluorometric Method
Sample type Paraffin section, frozen section, cell slide
Assay time 3 hours
Detection instrument Fluorescence Microscope
Dye Type Elab Fluor® 555
Ex/Em (nm) 555/565
Filter set TRITC
Other reagents required 4% Paraformaldehyde(E-IR-R113),0.2% Triton X-100(E-IR-R122),PBS(E-IR-R187),ddH2O,Mounting solution with anti-fluorescence quencher(E-IR-R119),Xylene,Ethanol
Storage -20°C, shading light
Shipping Ice bag
Expiration date 12 months

Operation Guide


  1. Biopreservation and Biobanking (2021) IF: 2.3
    Effects of AavLEA1 Protein on Mouse Ovarian Tissue Cryopreservation by Vitrification

    DOI: 10.1089/bio.2021.0048

    PMID: 34788107

  2. Molecular Genetics & Genomic Medicine (2022) IF: 2.473
    The compound heterozygous mutations of c.607G>a and c.657delC in the FAH gene are associated with renal damage with hereditary tyrosinemia type 1 (HT1)

    DOI: 10.1002/mgg3.2090

  3. Journal of Stroke & Cerebrovascular Diseases (2023) IF: 2.677
    Inhibition of PI3K/Akt/mTOR signaling by NDRG2 contributes to neuronal apoptosis and autophagy in ischemic stroke

    DOI: 10.1016/j.jstrokecerebrovasdis.2023.106984

  4. RENAL FAILURE (2022) IF: 3.222
    Rosiglitazone attenuates hypoxia-induced renal cell apoptosis by inhibiting NF-κB signaling pathway in a PPARγ-dependent manner

    DOI: 10.1080/0886022X.2022.2148539

  5. Biomedicines (2022) IF: 4.757
    Tauroursodeoxycholic Acid Reduces Neuroinflammation but Does Not Support Long Term Functional Recovery of Rats with Spinal Cord Injury

    DOI: 10.3390/biomedicines10071501

    PMID: 35884805

  6. DRUG DELIVERY (2022) IF: 6.419
    Sevelamer arsenite nanoparticle as a Pi-responsive drug carrier and embolic agent for chemoembolization

    DOI: 10.1080/10717544.2022.2072541

  7. Nature Communications (2022) IF: 17.694
    Long-term monitoring of intravital biological processes using fluorescent protein-assisted NIR-II imaging

    DOI: 10.1038/s41467-022-34274-w

    PMID: 36333308

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  • Q&A

A*********ySubmitted [ Jul 21 2023 ]

Asked: can I use this kit for bone tissue cartilage tissue? If not please sugest me a better one.



adminSubmitted [ Jul 21 2023 ]

Answered: Hello Anirban,thanks for your support.We will reply you via E-mail.


S*******eSubmitted [ Jul 20 2022 ]

Asked: Can someone explain how this kit can be used to visualize apoptosis in Drosophila melanogaster tissues?



adminSubmitted [ Jul 20 2022 ]

Answered: this kit can detect animal paraffin section, frozen section, cell slide. But insect sample is not verified in our lab. If you need to use insect samples for testing with this kit, I suggest to conduct pre-experiment to exploreteh sample preparation after consulting articles.

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