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One-step TUNEL In Situ Apoptosis Kit (Red, Elab Fluor^® 555)

Cat.No.:E-CK-A325

Applications:Apoptosis/DNA Fragmentation

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Detection principle

When cells undergo apoptosis, specific DNA endonucleases will be activated, cutting the genomic DNA between the nucleosomes. The DNA of apoptotic cells is cleaved into multimers of 180~200bp fragments, corresponding to the oligonucleosomal size. Therefore, the DNA of apoptotic cells typically migrates as a ladder of 180~200bp on an agarose gel. The exposed 3'-OH of the broken DNA can be catalyzed by Terminal Deoxynucleotidyl Transferase (TdT) with fluorescein labeled dUTP, which can be detected with fluorescence microscope.

General Information

Application	DNA Fragmentation
Detection method	Fluorometric Method
Sample type	Paraffin section, frozen section, cell slide
Assay time	3 hours
Detection instrument	Fluorescence Microscope
Dye Type	Elab Fluor^® 555
Ex/Em (nm)	555/565
Filter set	TRITC
Other reagents required	4% Paraformaldehyde(E-IR-R113),0.2% Triton X-100(E-IR-R122),PBS(E-IR-R187),ddH2O,Mounting solution with anti-fluorescence quencher(E-IR-R119),Xylene,Ethanol
Storage	-20°C, shading light
Shipping	Ice bag
Expiration date	12 months

Related Products

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E-CK-A301	Mitochondrial Membrane Potential Assay Kit (with JC-1)
E-CK-A311
E-CK-A362	Enhanced Cell Counting Kit 8 (WST-8/CCK8)

Operation Guide

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Reviews/Q&A

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Q&A

S*******eSubmitted [ Jul 20 2022 ]

Asked: Can someone explain how this kit can be used to visualize apoptosis in Drosophila melanogaster tissues?

adminSubmitted [ Jul 20 2022 ]

Answered: this kit can detect animal paraffin section, frozen section, cell slide. But insect sample is not verified in our lab. If you need to use insect samples for testing with this kit, I suggest to conduct pre-experiment to exploreteh sample preparation after consulting articles.

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