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When cells undergo apoptosis, specific DNA endonucleases will be activated, cutting the genomic DNA between the nucleosomes. The DNA of apoptotic cells is cleaved into multimers of 180~200bp fragments, corresponding to the oligonucleosomal size. Therefore, the DNA of apoptotic cells typically migrates as a ladder of 180~200bp on an agarose gel. The exposed 3'-OH of the broken DNA can be catalyzed by Terminal Deoxynucleotidyl Transferase (TdT) with fluorescein labeled dUTP, which can be detected with fluorescence microscope.
|Detection method||Fluorometric Method|
|Sample type||Paraffin section, frozen section, cell slide|
|Assay time||3 hours|
|Detection instrument||Fluorescence Microscope|
|Dye Type||Elab Fluor® 555|
|Other reagents required||4% Paraformaldehyde(E-IR-R113),0.2% Triton X-100(E-IR-R122),PBS(E-IR-R187),ddH2O,Mounting solution with anti-fluorescence quencher(E-IR-R119),Xylene,Ethanol|
|Storage||-20°C, shading light|
|Expiration date||12 months|
|E-CK-A321||One-step TUNEL In Situ Apoptosis Kit (Green, Elab Fluor® 488)|
|E-CK-A211||Annexin V-FITC/PI Apoptosis Kit|
|E-CK-A301||Mitochondrial Membrane Potential Assay Kit (with JC-1)|
|E-CK-A362||Enhanced Cell Counting Kit 8 (WST-8/CCK8)|
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S*******eSubmitted [ Jul 20 2022 ]
Asked: Can someone explain how this kit can be used to visualize apoptosis in Drosophila melanogaster tissues?
adminSubmitted [ Jul 20 2022 ]
Answered: this kit can detect animal paraffin section, frozen section, cell slide. But insect sample is not verified in our lab. If you need to use insect samples for testing with this kit, I suggest to conduct pre-experiment to exploreteh sample preparation after consulting articles.
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