One-step TUNEL In Situ Apoptosis Kit (Green, Elab Fluor^® 488) (E-CK-A321)

Name: One-step TUNEL In Situ Apoptosis Kit (Green, Elab Fluor® 488)
Brand: Elabscience
SKU: E-CK-A321
Price: 198.0 USD
Availability: InStock

Copy the product info.

Citations ({{ citationsPage.numTotal }}) Manual MSDS

Resources

One-step TUNEL In Situ Apoptosis Kit (Green, Elab Fluor<sup>®</sup> 488) - 1

Size	Price	Qty
100Assays	$ 498.00	- +
50Assays	$ 298.00	- +
20Assays	$ 198.00	- +

Bulk request

Add to cart

For research use only. Order now, ship in 3 days

Detection Principle	When cells undergo apoptosis, specific DNA endonucleases will be activated, cutting the genomic DNA between the nucleosomes. The DNA of apoptotic cells is cleaved into multimers of 180~200bp fragments, corresponding to the oligonucleosomal size. Therefore, the DNA of apoptotic cells typically migrates as a ladder of 180~200bp on an agarose gel. The exposed 3'-OH of the broken DNA can be catalyzed by Terminal Deoxynucleotidyl Transferase (TdT) with fluorescein labeled dUTP, which can be detected with fluorescence microscope.
Detection Method	Fluorometric method
Sample Type	Paraffin section;frozen section;cell slide
Assay Time	3 hours
Detection Instrument	Fluorescence Microscope
Dye Type	Elab Fluor®488
Ex/Em	495/519
Channel Set	FITC
Filter Set	FITC
Other Reagents Required	4% Paraformaldehyde(E-IR-R113),0.2% Triton X-100(E-IR-R122),PBS(E-BC-R187),ddH2O,Mounting solution with anti-fluorescence quencher(E-IR-R119),Xylene,Ethanol
Storage	This product can be stored at -20°C for 12 months with shading light.
Expiration date	12 months
Shipping	Ice bag

Cat.No.	Product Name	Sizes
E-CK-A211	Annexin V-FITC/PI Apoptosis Kit	200Assays , 100Assays , 50Assays , 20Assays
E-CK-A301	Mitochondrial Membrane Potential Assay Kit (with JC-1)	100Assays , 50Assays , 20Assays
E-CK-A320	One-step TUNEL In Situ Apoptosis Kit (Green, FITC)	100Assays , 50Assays , 20Assays
E-CK-A362	Enhanced Cell Counting Kit 8 (WST-8/CCK8)	500Tests , 100Tests