OVOL2 Polyclonal Antibody
Price: $ 399
Price: $ 240
Price: $ 143
Price: $ 73
- Host: Rabbit
- Reactivity: Human; Mouse
- Applications: WB;IHC
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Verified Samples |
Verified Samples in WB:Human cerebella,Human cerebrum,Human skin,NIH/3T3,SKOV3 Verified Samples in IHC:Human esophagus cancer |
Dilution |
WB 1:500-1:2000, IHC 1:30-1:150 Western Blot Operation Guide |
Clonality | Polyclonal |
Immunogen | Fusion protein of human OVOL2 |
Abbre | OVOL2 |
Synonyms | bA504H3.3;EUROIMAGE566589;hOvo 2;hOvo2;Ovo like 2 (Drosophila);Ovo like 2;ovo-like zinc finger 2;OVOL 2;OVOL2;OVOL2;Transcription factor Ovo like 2;Transcription factor Ovo-like 2;Zinc finger protein 339;ZNF 339;ZNF339 |
Swissprot | |
Calculated MW | 30 kDa |
Observed MW |
Refer to figures
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
Cellular Localization | Nucleus. |
Concentration | 0.84 mg/mL |
Buffer | PBS with 0.05% NaN3 and 40% Glycerol,pH7.4 |
Purification Method | Antigen affinity purification |
Research Areas | Epigenetics and Nuclear Signaling; Developmental biology; Stem cells |
Conjugation | Unconjugated |
Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
Shipping | Ice bag |
background | This gene encodes a member of the evolutionarily conserved ovo-like protein family. Mammalian members of this family contain a single zinc finger domain composed of a tetrad of C2H2 zinc fingers with variable N- and C-terminal extensions that contain intrinsically disordered domains. Members of this family are involved in epithelial development and differentiation. Knockout of this gene in mouse results in early embryonic lethality with phenotypes that include neurectoderm expansion, impaired vascularization, and heart anomalies. In humans, allelic variants of this gene have been associated with posterior polymorphous corneal dystrophy. |