Synonyms:Antigen NY-CO-13,BCC7,Cellular tumor antigen p53,FLJ92943,LFS1,Mutant tumor protein 53,p53,p53 tumor suppressor,P53,Phosphoprotein p53,Tp53,Transformation related protein 53,TRP53,Tumor protein 53,Tumor protein p53,Tumor suppressor p53
|Synonyms||Antigen NY-CO-13,BCC7,Cellular tumor antigen p53,FLJ92943,LFS1,Mutant tumor protein 53,p53,p53 tumor suppressor,P53,Phosphoprotein p53,Tp53,Transformation related protein 53,TRP53,Tumor protein 53,Tumor protein p53,Tumor suppressor p53|
|Application||WB(Detection kit: E-IR-R304),IHC-p(Detection kit: E-IR-R217),IF|
|Recommended dilution||WB 1:500-1:2000, IHC 1:100-1:300, IF 1:100-1:200|
|Cellular localization||Cytoplasm; Cytoplasm. Nucleus. Nucleus>PML body. Endoplasmic reticulum. Interaction with BANP promotes nuclear localization. Recruited into PML bodies together with CHEK2; Nucleus. Cytoplasm. Localized in both nucleus and cytoplasm in most cells. In some cells, forms foci in the nucleus that are different from nucleoli; Nucleus. Cytoplasm. Localized in the nucleus in most cells but found in the cytoplasm in some cells; Nucleus. Cytoplasm. Localized mainly in the nucleus with minor staining in the cytoplasm; Nucleus. Cytoplasm. Predominantly nuclear but localizes to the cytoplasm when expressed with isoform 4 and Nucleus. Cytoplasm. Predominantly nuclear but translocates to the cytoplasm following cell stress.|
|Tissue specificity||Ubiquitous. Isoforms are expressed in a wide range of normal tissues but in a tissue-dependent manner. Isoform 2 is expressed in most normal tissues but is not detected in brain, lung, prostate, muscle, fetal brain, spinal cord and fetal liver. Isoform 3 is expressed in most normal tissues but is not detected in lung, spleen, testis, fetal brain, spinal cord and fetal liver. Isoform 7 is expressed in most normal tissues but is not detected in prostate, uterus, skeletal muscle and breast. Isoform 8 is detected only in colon, bone marrow, testis, fetal brain and intestine. Isoform 9 is expressed in most normal tissues but is not detected in brain, heart, lung, fetal liver, salivary gland, breast or intestine.|
|Purification||Protein A purification|
|Storage instructions||Store at -20℃. Avoid freeze / thaw cycles.|
|Storage buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.4.|
|Background||Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. Implicated in Notch signaling cross-over. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis.|
Western Blot analysis of 293T cells using p53 Monoclonal Antibody at dilution of 1) 1:1000 2) 1:2000.
Immunohistochemistry of paraffin-embedded Human colon cancer tissue using p53 Monoclonal Antibody at dilution of 1:200.
Immunofluorescence analysis of Hela tissue using p53 Monoclonal Antibody at dilution of 1:100.