PBS Buffer, pH7.4 (10 ×)

    • PBS Buffer, pH7.4(10 ×)-Elabscience
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    • PBS Buffer, pH7.4(10 ×)-Elabscience
    • PBS Buffer, pH7.4(10 ×)-Elabscience

      Catalog number:E-BC-R187

      Size:
      • 100 mL
      • 200 mL
      • 500 mL
      Qty:
      - +
      Price: $10

      Application: WB

      Storage: RT, 6 months

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request Manual

      Introduction

      PBS buffer is a phosphate buffer, which provides a relatively stable ionic environment and pH buffering capacity and is the most commonly used buffer for biochemistry. When you prepare the WB samples, pre-cooled PBS are used to wash the surface of tissues and internal debris or cell culture medium to prevent the interference of endogenous IgG or culture medium.

       

      Components

       

      Products

      100 mL

      200 mL

      500 mL

      PBS Buffer, pH7.4 (10 ×)

      1 × 100 mL

      2 × 100 mL

      2 × 250 mL

      Manual

      1 copy



      Instructions

      This product is a 10 × concentrated solution, dilute with pure water to 1 × working solution before use.

      For example: take 100 mL PBS buffer (10 × ), dilute with pure water to 1 L.


      1 × PBS buffer component

      136.9 mM NaCl, 2.68 mM KCl, 10 mM Na2HPO4•12H2O, 2 mM KH2PO4, pH 7.4.

       

      Storage

      Store at room temperature for 6 months.


      Cautions

      1. PBS may precipitate when stored at low temperature. If PBS precipitates, please dissolve PBS in water bath at 37℃, and then dilute it to 1 x working buffer.

      2. Keep the product sealed to prevent from pollution.

      3. For your safety and health, please wear the lab coat and disposable gloves before the experiments.

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