PDGFR beta Polyclonal Antibody (E-AB-67422)
For research use only.
| Verified Samples |
Verified Samples in WB:SH-SY5Y, Mouse lung, NIH/3T3,C2C12 Verified Samples in IHC: Rat kidney, Human placenta Verified Samples in IF: C6, NIH/3T3 |
| Dilution | WB 1:50-1:2000, IHC-P 1:50-1:200, IF/ICC 1:50-1:200, ELISA Recommended starting concentration is 1 μg/mL. |
| Isotype | Rabbit IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC-P, IF/ICC, ELISA |
| Clonality | Polyclonal |
| Immunogen | Recombinant fusion protein of human PDGFR beta (NP_002600.1). |
| Abbre | PDGFR beta |
| Synonyms | CD140B, IBGC4, IMF1, JTK12, KOGS, PDGF Receptor beta, PDGFR, PDGFR beta, PDGFR-1, PDGFR1, PDGFRB, PENTT |
| Swissprot | |
| Calculated MW | 124 kDa |
| Observed MW |
200 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cell membrane, Cytoplasmic vesicle, Lysosome lumen, Single-pass type I membrane protein. |
| Concentration | 1 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer and 50% glycerol. |
| Purification Method | Affinity purification |
| Research Areas | Cancer, Cardiovascular, Signal Transduction |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | The protein encoded by this gene is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer (PDGFB or PDGFD) or a heterodimer (PDGFA and PDGFB). This gene is essential for normal development of the cardiovascular system and aids in rearrangement of the actin cytoskeleton. This gene is flanked on chromosome 5 by the genes for granulocyte-macrophage colony-stimulating factor and macrophage-colony stimulating factor receptor; all three genes may be implicated in the 5-q syndrome. A translocation between chromosomes 5 and 12, that fuses this gene to that of the ETV6 gene, results in chronic myeloproliferative disorder with eosinophilia. |
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