Current Position:Home>>Antibodies>>Flow Cytometry Antibodies>> PE Anti-Human CD273/PD-L2 Antibody[24F.10C12]
Cat.No.:E-AB-F1175D
Welcome to order from local distributors.
FAQ for Flow Cytometry Antibody
Add to cart Bulk requestFor research use only. Order now, ship in 3 days
Alternate Names | B7DC, CD273, PDCD1L2, PDL2 |
Swissprot | Q9BQ51 |
References |
1. Brown JA, et al. 2003. J. Immunol. 170:1257
2. Rodig N, et al. 2003. Eur. J. Immunol. 33:3117 |
Form | Liquid |
Clone No. | 24F.10C12(See Other Available Formats) |
Host | Mouse |
Isotype | Mouse IgG2a, κ |
Isotype Control | PE Mouse IgG2a, κ Isotype Control[C1.18.4] [Product E-AB-F09802D] |
Reactivity | Human |
Application | FCM (See the Application Description) |
Storage Buffer | Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant. |
Recommended Use | Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. |
Shipping | Biological ice pack at 4℃ |
Stability & Storage | Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase. |
Conjugation | PE |
PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 585/42 nm bandpass filter). | |
How to select the appropriate detection channel through the spectrogram?
CD273, known as B7-DC, is also called programmed death ligand 2 (PDL2). This ligand is a 25 kD type I transmembrane protein and a member of B7 family within the immunoglobulin receptor superfamily and is expressed on a subset of dendritic cells, liver and a small subset of macrophages as well as a few transformed cell lines. CD273 has been reported to be stimulatory on dendritic cells when cross-linked and to inhibit T cell activation upon engaging the PD-1 receptor. CD273 has also been reported to bind to an alternative receptor and to mediate T cell activation through such non-PD1 mediated interactions. |
Related Protocols | Staining Cell Surface Targets for Flow Cytometry |
Troubleshooting |
Product Name | Sizes | Price |
APC Anti-Human CD273/PD-L2 Antibody[24F.10C12] | 20Tests, 100Tests, 100Tests×2 | Check More |