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PE Anti-Human CD273/PD-L2 Antibody[24F.10C12]

Uniprot : Q9BQ51
  • Cat.No.:E-AB-F1175D

  • Applications: FCM
  • Isotype: Mouse IgG2a, κ
  • Host: Mouse

To Purchase E-AB-F1175D

Size:
  • 20Tests
  • 100Tests
  • 100Tests×2
Price: $90
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Antigen Details

Alternate Names B7DC, CD273, PDCD1L2, PDL2
Swissprot Q9BQ51
References
1. Brown JA, et al. 2003. J. Immunol. 170:1257
2. Rodig N, et al. 2003. Eur. J. Immunol. 33:3117

Product Details

Form Liquid
Clone No. 24F.10C12(See Other Available Formats)
Host Mouse
Isotype Mouse IgG2a, κ
Isotype Control PE Mouse IgG2a, κ Isotype Control[C1.18.4] [Product E-AB-F09802D]
Reactivity Human
Application FCM (See the Application Description)
Storage Buffer Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping Biological ice pack at 4℃
Stability & Storage Keep as concentrated solution.
Store at 2~8°C and protected from prolonged exposure to light. Do not freeze.
Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.
Conjugation PE
PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 585/42 nm bandpass filter).


How to select the appropriate detection channel through the spectrogram?

Background

CD273, known as B7-DC, is also called programmed death ligand 2 (PDL2). This ligand is a 25 kD type I transmembrane protein and a member of B7 family within the immunoglobulin receptor superfamily and is expressed on a subset of dendritic cells, liver and a small subset of macrophages as well as a few transformed cell lines. CD273 has been reported to be stimulatory on dendritic cells when cross-linked and to inhibit T cell activation upon engaging the PD-1 receptor. CD273 has also been reported to bind to an alternative receptor and to mediate T cell activation through such non-PD1 mediated interactions.

Protocols & Troubleshootings

Related Protocols Staining Cell Surface Targets for Flow Cytometry
Troubleshooting

Troubleshooting for Flow Cytometry

Alternative Formats

Product Name Sizes Price
APC Anti-Human CD273/PD-L2 Antibody[24F.10C12] 20Tests, 100Tests, 100Tests×2 Check More

Citations

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