Alternate Names	Leukocyte surface antigen CD47,Cd47,Integrin-associated protein,IAP
Swissprot	Q08722
References	1. Anstee DJ, et al. 1995. In Leucocyte Typing V (Schlossman ed.) Oxford University Press Oxford pp233-234. 2. Brown E, et al. 1990. J. Cell Biol. 111:2785. 3. Gao AG, et al. 1996. J. Biol. Chem. 271:21. 4. Lindberg FP, et al. 1994. J. Biol. Chem. 269:1567.

Product Details

Form	Liquid
Clone No.	CC2C6D4
Host	Mouse
Isotype	Mouse IgG1, κ
Isotype Control	PE Mouse IgG1, κ Isotype Control[MOPC-21] [Product E-AB-F09792D]
Reactivity	Human
Application	FCM
Storage Buffer	Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use	Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping	Biological ice pack at 4℃
Stability & Storage	Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Conjugation	PE
	PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 585/42 nm bandpass filter).

How to select the appropriate detection channel through the spectrogram?

Background

CD47 also known as Rh-associated protein, gp42, integrin-associated protein (IAP), and neurophilin, is a 42-52 kD member of the immunoglobulin superfamily containing a five-pass transmembrane attachment. Two splice variants have been described in the cytoplasmic tail, the shorter form is expressed in bone-marrow-derived cells, endothelial cells, and fibroblasts while the longer form is expressed by neural tissues. CD47 expression is widely distributed in hematopoietic cells including thymocytes, T cells, B cells, monocytes, platelets, and erythrocytes as well as epithelial cells, endothelial cells, fibroblasts, and neural tissues. CD47 functions as an adhesion molecule and thrombospondin receptor and is non-covalently associated with β3 integrins CD51/CD61, CD41/CD61. Thrombospondin is a ligand for CD47; in the absence of CD47 mice show defects in host defense and β3 integrin-dependent ligand binding, migration, and cellular activation. CD47 is also part of the Rh complex on erythrocytes.

Protocols & Troubleshootings

Related Protocols	Staining Cell Surface Targets for Flow Cytometry
Troubleshooting	Troubleshooting for Flow Cytometry

Alternative Formats

Product Name	Sizes	Price
Purified Anti-Human CD47 Antibody[CC2C6D4]	25μg, 100μg	Check More
Biotin Anti-Human CD47 Antibody[CC2C6D4]	25μg, 100μg	Check More
FITC Anti-Human CD47 Antibody[CC2C6D4]	20Tests, 100Tests, 100Tests×2	Check More
APC Anti-Human CD47 Antibody[CC2C6D4]	20Tests, 100Tests, 100Tests×2	Check More
PerCP Anti-Human CD47 Antibody[CC2C6D4]	20Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5 Anti-Human CD47 Antibody[CC2C6D4]	20Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine7 Anti-Human CD47 Antibody[CC2C6D4]	20Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5.5 Anti-Human CD47 Antibody[CC2C6D4]	20Tests, 100Tests, 100Tests×2	Check More
PerCP/Cyanine5.5 Anti-Human CD47 Antibody[CC2C6D4]	20Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 488 Anti-Human CD47 Antibody[CC2C6D4]	20Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 647 Anti-Human CD47 Antibody[CC2C6D4]	20Tests, 100Tests, 100Tests×2	Check More
PE/Elab Fluor^® 594 Anti-Human CD47 Antibody[CC2C6D4]	20Tests, 100Tests, 100Tests×2	Check More

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