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PE Anti-Human IL-10 Antibody[JES3-9D7]

Uniprot : P22301
  • Cat.No.:E-AB-F1198D

  • Applications: ICFCM
  • Isotype: Rat IgG1, κ
  • Host: Rat

To Purchase E-AB-F1198D

Size:
  • 20Tests
  • 100Tests
  • 100Tests×2
Price: $75
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Antigen Details

Alternate Names Interleukin-10,IL-10,Cytokine synthesis inhibitory factor,CSIF
Swissprot P22301
Gene ID 3586
References
1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. de Waal-Malefyt R, et al. 1992. Curr. Opin. Immunol. 4:314.
3. Howard M, et al. 1992. Immunol. Today. 13:198.
4. Quesniaux V. 1992. Research Immunol. 143:385.

Product Details

Form Liquid
Clone No. JES3-9D7(See Other Available Formats)
Host Rat
Isotype Rat IgG1, κ
Isotype Control PE Rat IgG1, κ Isotype Control[HRPN] [Product E-AB-F09822D]
Reactivity Human
Application ICFCM (See the Application Description)
Storage Buffer Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping Biological ice pack at 4℃
Stability & Storage Keep as concentrated solution.
Store at 2~8°C and protected from prolonged exposure to light. Do not freeze.
Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.
Conjugation PE
PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 585/42 nm bandpass filter).


How to select the appropriate detection channel through the spectrogram?

Background

IL-10 was originally described as Cytokine Synthesis Inhibitory Factor (CSIF) by virtue of its ability to inhibit cytokine production by Th1 clones. IL-10 shares over 80% sequence homology with the Epstein-Barr virus protein BCRFI. The biological activities of IL-10 include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayed type hypersensitivity response, and stimulation of the Th2 cell response, which results in elevated antibody production.

Protocols & Troubleshootings

Related Protocols Staining Intracellular Antigens for Flow Cytometry
Troubleshooting

Troubleshooting for Flow Cytometry

Alternative Formats

Product Name Sizes Price
FITC Anti-Human IL-10 Antibody[JES3-9D7] 20Tests, 100Tests, 100Tests×2 Check More
APC Anti-Human IL-10 Antibody[JES3-9D7] 20Tests, 100Tests, 100Tests×2 Check More
Elab Fluor® 488 Anti-Human IL-10 Antibody[JES3-9D7] 20Tests, 100Tests, 100Tests×2 Check More
Elab Fluor® 647 Anti-Human IL-10 Antibody[JES3-9D7] 20Tests, 100Tests, 100Tests×2 Check More

Citations

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