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Cat.No.:E-AB-F1205D
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Alternate Names | Interleukin-5,IL-5,B-cell differentiation factor I,T-cell replacing factor,TRF |
Swissprot | P05113,P04401 |
References |
1. Fitzgerald, K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Takatsu, K, et al. 1988. Immunol. Rev. 102:107. 3. Takatsu, K. 1992. Curr. Opin. Immunol. 4:299. 4. Takatsu, K. 1991. Microbiol. Immunol. 35:593. |
Form | Liquid |
Clone No. | TRFK5(See Other Available Formats) |
Host | Rat |
Isotype | Rat IgG1, κ |
Isotype Control | PE Rat IgG1, κ Isotype Control[HRPN] [Product E-AB-F09822D] |
Reactivity | Human,Mouse |
Application | ICFCM (See the Application Description) |
Storage Buffer | Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant. |
Recommended Use | Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. |
Shipping | Biological ice pack at 4℃ |
Stability & Storage | Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase. |
Conjugation | PE |
PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 585/42 nm bandpass filter). | |
How to select the appropriate detection channel through the spectrogram?
IL-5 is a homodimeric, disulphide-linked protein produced by T-cells. Monomeric human IL-5 is a 126 amino acid protein with a reported molecular weight of 26 kD for the homodimeric protein. Mouse and human IL-5 are approximately 70% identical. IL-5 has been shown to promote the growth of immature hematopoietic BFU-E progenitors, stimulate the activation and differentiation of eosinophils, and promote the generation of cytotoxic lymphocytes. Mouse IL-5 induces the differentiation and proliferation of pre-activated B-cells and stimulates the production and secretion of IgM and IgA by B-cells stimulated with bacterial endotoxin. The TRFK5 antibody can neutralize the bioactivity of natural or recombinant IL-5. |
Related Protocols | Staining Intracellular Antigens for Flow Cytometry |
Troubleshooting |
Product Name | Sizes | Price |
AF/LE Purified Anti-Human/Mouse IL-5 Antibody[TRFK5] | 50μg, 500μg, 1mg | Check More |
Purified Anti-Human/Mouse IL-5 Antibody[TRFK5] | 25μg, 100μg | Check More |
APC Anti-Human/Mouse IL-5 Antibody[TRFK5] | 20Tests, 100Tests, 100Tests×2 | Check More |
PE Anti-Human/Mouse IL-5 Antibody[TRFK5] | 25μg, 100μg | Check More |
APC Anti-Human/Mouse IL-5 Antibody[TRFK5] | 25μg, 100μg | Check More |