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Cat.No.:E-AB-F1177D
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Alternate Names | Slam,Slamf1,CD 150,Signaling lymphocytic activation molecule |
Swissprot | Q9QUM4 |
References |
1. Cocks BG, et al. 1995. Nature 376:260.
2. Punnonen J, et al. 1997. J. Exp. Med. 185:993. 3. Sidorenko SP, et al. 1993. J. Immunol. 151:4614. |
Form | Liquid |
Clone No. | TC15-12F12.2(See Other Available Formats) |
Host | Rat |
Isotype | Rat IgG2a, λ |
Isotype Control | PE Rat IgG2a, λ Isotype Control[B39-4] [Product E-AB-F09742D] |
Reactivity | Mouse |
Application | FCM (See the Application Description) |
Storage Buffer | Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant. |
Recommended Use | Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. |
Shipping | Biological ice pack at 4℃ |
Stability & Storage | Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase. |
Conjugation | PE |
PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 585/42 nm bandpass filter). | |
How to select the appropriate detection channel through the spectrogram?
CD150 is a 75-95 kD member of the immunoglobulin superfamily, also known as SLAM (signaling lymphocyte activation molecule) or IPO-3. CD150, a single chain type I transmembrane molecule, is expressed on thymocytes, T cell subsets, B cells, dendritic cells, and endothelial cells. The expression is upregulated upon activation. CD150 expression has been shown to be maintained on Th1 but not Th2 clones. T regulatory cells express a relatively high level of CD150. Antibodies against CD150 have been shown to augment IFN-γ production by Th1 cells, especially when co-stimulated through the TCR. CD150 associates with the src homology 2-domain-containing protein tyrosine phosphatase SHP-2, and this association is thought to be involved in signal transduction. In combination with CD48, CD150 is a useful marker for hematopoietic stem cell studies. |
Related Protocols | Staining Cell Surface Targets for Flow Cytometry |
Troubleshooting |
Product Name | Sizes | Price |
FITC Anti-Mouse CD150/SLAM Antibody[TC15-12F12.2] | 50Tests, 100Tests, 100Tests×2 | Check More |
APC Anti-Mouse CD150/SLAM Antibody[TC15-12F12.2] | 50Tests, 100Tests, 100Tests×2 | Check More |
PE Anti-Mouse CD150/SLAM Antibody[TC15-12F12.2] | 25μg, 100μg | Check More |
APC Anti-Mouse CD150/SLAM Antibody[TC15-12F12.2] | 25μg, 100μg | Check More |