Alternate Names	CD16a/b,CD32,CD32A/B,Fc fragment of IgG low affinity IIa/b receptor,Fc fragment of IgG low affinity IIIa/b receptor,Fc fragment of IgG low affinity IIIb receptor,Fc gamma receptor III A/B,FCG2A,FcGR,FCGR2A/BFCGR3,FCGR3A/B,Fc gamma RIIa/b
Swissprot	P08508,P08101
References	1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Unkeless JC. 1989. J. Clin. Invest. 83:355. 3. Qiu WQ, et al. 1990. Science 248:732.

Product Details

Form	Liquid
Clone No.	2.4G2(See Other Available Formats)
Host	Rat
Isotype	Rat IgG2b, κ
Isotype Control	PE Rat IgG2b, κ Isotype Control[LTF-2] [Product E-AB-F09843D]
Reactivity	Mouse
Application	FCM (See the Application Description)
Storage Buffer	Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use	Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/10⁶ cells in 100 μL volume].
Shipping	Biological ice pack at 4℃
Stability & Storage	Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Conjugation	PE
	PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 585/42 nm bandpass filter).

How to select the appropriate detection channel through the spectrogram?

Background

CD16 is low affinity IgG Fc receptor III (FcR III) and CD32 is FcR II. CD16/CD32 are expressed on B cells, monocytes/macrophages, NK cells, granulocytes, mast cells, and dendritic cells. The Fc receptors bind antibody-antigen immune complexes and mediate adaptive immune responses.

Protocols & Troubleshootings

Related Protocols	Staining Cell Surface Targets for Flow Cytometry
Troubleshooting	Troubleshooting for Flow Cytometry

Alternative Formats

Product Name	Sizes	Price
AF/LE Purified Anti-Mouse CD16/32 Antibody[2.4G2]	50μg, 500μg, 1mg	Check More
Purified Anti-Mouse CD16/32 Antibody[2.4G2]	25μg, 100μg	Check More
Biotin Anti-Mouse CD16/32 Antibody[2.4G2]	25μg, 100μg	Check More
FITC Anti-Mouse CD16/32 Antibody[2.4G2]	50Tests, 100Tests, 100Tests×2	Check More
PE Anti-Mouse CD16/32 Antibody[2.4G2]	50Tests, 100Tests, 100Tests×2	Check More
APC Anti-Mouse CD16/32 Antibody[2.4G2]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5 Anti-Mouse CD16/32 Antibody[2.4G2]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine7 Anti-Mouse CD16/32 Antibody[2.4G2]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5.5 Anti-Mouse CD16/32 Antibody[2.4G2]	50Tests, 100Tests, 100Tests×2	Check More
PerCP/Cyanine5.5 Anti-Mouse CD16/32 Antibody[2.4G2]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 488 Anti-Mouse CD16/32 Antibody[2.4G2]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 647 Anti-Mouse CD16/32 Antibody[2.4G2]	50Tests, 100Tests, 100Tests×2	Check More
PE/Elab Fluor^® 594 Anti-Mouse CD16/32 Antibody[2.4G2]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® Violet 450 Anti-Mouse CD16/32 Antibody[2.4G2]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® Red 780 Anti-Mouse CD16/32 Antibody[2.4G2]	50Tests, 100Tests, 100Tests×2	Check More
FITC Anti-Mouse CD16/32 Antibody[2.4G2]	25μg, 100μg	Check More
APC Anti-Mouse CD16/32 Antibody[2.4G2]	25μg, 100μg	Check More
PE/Cyanine5 Anti-Mouse CD16/32 Antibody[2.4G2]	25μg, 100μg	Check More
PE/Cyanine7 Anti-Mouse CD16/32 Antibody[2.4G2]	25μg, 100μg	Check More
PE/Cyanine5.5 Anti-Mouse CD16/32 Antibody[2.4G2]	25μg, 100μg	Check More
PerCP/Cyanine5.5 Anti-Mouse CD16/32 Antibody[2.4G2]	25μg, 100μg	Check More
Elab Fluor^® 488 Anti-Mouse CD16/32 Antibody[2.4G2]	25μg, 100μg	Check More
Elab Fluor^® 647 Anti-Mouse CD16/32 Antibody[2.4G2]	25μg, 100μg	Check More
PE/Elab Fluor^® 594 Anti-Mouse CD16/32 Antibody[2.4G2]	25μg, 100μg	Check More
Elab Fluor^® Violet 450 Anti-Mouse CD16/32 Antibody[2.4G2]	25μg, 100μg	Check More
Elab Fluor^® Red 780 Anti-Mouse CD16/32 Antibody[2.4G2]	25μg, 100μg	Check More

Citations

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