Current Position:Home>>Antibodies>>Flow Cytometry Antibodies>> PE Anti-Mouse CD1d Antibody[19G11] E-AB-F1032UD
Cat.No.:E-AB-F1032UD
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Alternate Names | Antigen-presenting glycoprotein CD1d1,Cd1d1,CD1d.1,Cd1d1,Cd1.1 |
Swissprot | P11609 |
References |
1. Arrenberg P, et al. 2010. P. Natl. Acad. Sci. USA 107:10984.
2. Mattarollo SR, et al. 2010. J. Immunol. 184:5663. 3. Wang J, et al. 2010. P. Natl. Acad. Sci. USA 107:1535. 4. Nieuwenhuis EE, et al. 2009. J. Clin. Invest. 119:1241. |
Form | Liquid |
Clone No. | 19G11(See Other Available Formats) |
Host | Rat |
Isotype | Rat IgG2b, κ |
Isotype Control | PE Rat IgG2b, κ Isotype Control[LTF-2] [Product E-AB-F09843D] |
Reactivity | Mouse |
Application | FCM (See the Application Description) |
Storage Buffer | Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant. |
Recommended Use | Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/106 cells in 100 μL volume]. |
Shipping | Biological ice pack at 4℃ |
Stability & Storage | Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase. |
Conjugation | PE |
PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 585/42 nm bandpass filter). | |
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How to select the appropriate detection channel through the spectrogram?
CD1d is a type I transmembrane protein and member of the MHC family, with a molecular weight ranging from 43-49 kD, depending on the glycosylation degree. CD1d is expressed by antigen presenting cells such as dendritic cells, monocytes, macrophages and B cells; also expressed by thymocytes and intestinal epithelial cells. CD1d present glycolipids to iNKT cells that recognize them by their Vα14i TCR. |
Related Protocols | Staining Cell Surface Targets for Flow Cytometry |
Troubleshooting |
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