Alternate Names	T-cell-specific surface glycoprotein CD28,Cd28,CD28
Swissprot	P31041
References	1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Lenschow DJ, et al. 1996. Annu. Rev. Immunol. 14:233. 3. Gross JA, et al. 1992. J. Immunol. 149:380.

Product Details

Form	Liquid
Clone No.	37.51(See Other Available Formats)
Host	Syrian Hamster
Isotype	Syrian Hamster IgG
Isotype Control	PE Syrian Hamster IgG Isotype Control[SHG-1] [Product E-AB-F09763D]
Reactivity	Mouse
Application	FCM (See the Application Description)
Storage Buffer	Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use	Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/10⁶ cells in 100 μL volume].
Shipping	Biological ice pack at 4℃
Stability & Storage	Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Conjugation	PE
	PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 585/42 nm bandpass filter).

How to select the appropriate detection channel through the spectrogram?

Background

CD28 is a 44 kD glycoprotein, also known as Tp44 or T44. It is a member of the Ig superfamily, expressed on thymocytes, most peripheral T cells, and NK cells. In association with CD80 (B7-1) and CD86 (B7-2), CD28 acts as the second signal for T and NK cell activation and proliferation. The 37.51 antibody has been reported to augment in vitro T cell proliferation and cytokine production, and promote CTL development.

Protocols & Troubleshootings

Related Protocols	Staining Cell Surface Targets for Flow Cytometry
Troubleshooting	Troubleshooting for Flow Cytometry

Alternative Formats

Product Name	Sizes	Price
AF/LE Purified Anti-Mouse CD28 Antibody[37.51]	50μg, 500μg, 1mg	Check More
Purified Anti-Mouse CD28 Antibody[37.51]	25μg, 100μg	Check More
PE Anti-Mouse CD28 Antibody[37.51]	50Tests, 100Tests, 100Tests×2	Check More
APC Anti-Mouse CD28 Antibody[37.51]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 488 Anti-Mouse CD28 Antibody[37.51]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 647 Anti-Mouse CD28 Antibody[37.51]	50Tests, 100Tests, 100Tests×2	Check More
APC Anti-Mouse CD28 Antibody[37.51]	25μg, 100μg	Check More
Elab Fluor^® 488 Anti-Mouse CD28 Antibody[37.51]	25μg, 100μg	Check More
Elab Fluor^® 647 Anti-Mouse CD28 Antibody[37.51]	25μg, 100μg	Check More

Citations

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