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Cat.No.:E-AB-F1284UD
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Alternate Names | Hematopoietic progenitor cell antigen CD34 |
Swissprot | Q64314 |
Gene ID | 12490 |
References |
1. Akashi K, et al. 2000. Nature. 404(6774):193-197.
2. Brown J, et al. 1991. Int Immunol. 1991; 3(2):175-184. 3. Goodell MA, et al. 1997. Nat Med. 404(6774):193-197. 4. Lu J, et al. 1999. Exp Hematol. 27(2):272-281. 5. Morel F, et al. 1996. Blood. 88(10):3774-3784. 6. Osawa M, et al. 1996. Science. 273(5272):242-245. 7. Spangrude GJ, et al. 1988. Science. 241(4861):58-62. 8. Suda J, et al. 1992. Blood. 79(9):2288-2295. 9. Suzuki A, et al. 1996. Blood. 87(9):3550-3562. |
Form | Liquid |
Clone No. | RAM34(See Other Available Formats) |
Host | Rat |
Isotype | Rat IgG2a, κ |
Isotype Control | PE Rat IgG2a, κ Isotype Control[2A3] [Product E-AB-F09833D] |
Reactivity | Mouse |
Application | FCM (See the Application Description) |
Storage Buffer | Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant. |
Recommended Use | Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/106 cells in 100 μL volume]. |
Shipping | Biological ice pack at 4℃ |
Stability & Storage | Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase. |
Conjugation | PE |
PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 585/42 nm bandpass filter). | |
How to select the appropriate detection channel through the spectrogram?
The RAM34 antibody reacts with the CD34 glycoprotein on the surface of three independently derived mouse CD34-transfected cell lines. RAM34 antibody also reacts with the mouse cell lines PA6, 416B, Swiss 3T6, NIH, 3T3, DA1, and M1, all of which are positive for expression of mouse CD34 mRNA. Cell lines shown to be negative for CD34 transcript, including WEHI-3B, EL4, 18.8, and CMT64/61, are also negative for surface expression of CD34 as determined by RAM34 staining. Normal thymocytes and splenocytes are negative for CD34 expression. In the bone marrow, 7-10% of cells are stained with RAM34 mAb, including most of the Ly-6A/E (Sca-1)+ CD90 (Thy-1)low Lineage Marker- hematopoietic stem cell-enriched subpopulation and myeloerythroid progenitors. CD34 is also expressed on a small percentage of fetal liver cells, including NK-cell progenitors. CD34 has been reported to be expressed on the endothelium of capillaries and, in this form, to function as a ligand for L-selectin. Consistent with this observation, RAM34 antibody stains endothelial cells in spleen, thymus, and postcapillary HEVs in the lymph nodes. It is reported that RAM34 antibody can be used to select CD34+ CD117 (c-Kit)+ Ly-6A/E (Sca-1)+ Lineage Marker- bone marrow-derived hematopoietic stem cells, capable of short-term multi-lineage reconstitution of lethally irradiated mice; while the CD34- CD117+ Sca-1+ Lineage Marker- population contains self-renewing hematopoietic stem cells. Similarly, the bone marrow population with high dye-efflux capacity and which is highly enriched for long-term reconstituting hematopoietic stem cells is CD34- CD117 (c-Kit)+ Ly-6A/E (Sca-1)+ Lineage Marker-. |
Related Protocols | Staining Cell Surface Targets for Flow Cytometry |
Troubleshooting |
Product Name | Sizes | Price |
PE Anti-Mouse CD34 Antibody[RAM34] | 50Tests, 100Tests, 100Tests×2 | Check More |
APC Anti-Mouse CD34 Antibody[RAM34] | 50Tests, 100Tests, 100Tests×2 | Check More |
APC Anti-Mouse CD34 Antibody[RAM34] | 25μg, 100μg | Check More |