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PE Anti-Mouse CD45.2 Antibody[104.2]

  • Cat.No.:E-AB-F1122D

  • Applications: FCM
  • Isotype: Mouse IgG2a, κ
  • Host: Mouse

To Purchase E-AB-F1122D

Size:
  • 50Tests
  • 100Tests
  • 100Tests×2
Price: $20
Qty:

Antigen Details

Alternate Names Ly-5.2, LCA
References
1. Suzuki K, et al. 2000. Immunity 13:691.

Product Details

Form Liquid
Clone No. 104.2
Host Mouse
Isotype Mouse IgG2a, κ
Isotype Control PE Mouse IgG2a, κ Isotype Control[C1.18.4] [Product E-AB-F09802D]
Reactivity Mouse
Application FCM
Storage Buffer Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping Biological ice pack at 4℃
Stability & Storage Keep as concentrated solution.
Store at 2~8°C and protected from prolonged exposure to light. Do not freeze.
Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.
Conjugation PE
PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 585/42 nm bandpass filter).


How to select the appropriate detection channel through the spectrogram?

Background

CD45.2 is an alloantigen of CD45, expressed by Ly5.2 bearing mouse strains (e.g., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in the mouse that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Protocols & Troubleshootings

Related Protocols Staining Cell Surface Targets for Flow Cytometry
Troubleshooting

Troubleshooting for Flow Cytometry

Alternative Formats

Product Name Sizes Price
AF/LE Purified Anti-Mouse CD45.2 Antibody[104.2] 50μg, 500μg, 1mg Check More
Purified Anti-Mouse CD45.2 Antibody[104.2] 25μg, 100μg Check More
Biotin Anti-Mouse CD45.2 Antibody[104.2] 25μg, 100μg Check More
FITC Anti-Mouse CD45.2 Antibody[104.2] 50Tests, 100Tests, 100Tests×2 Check More
APC Anti-Mouse CD45.2 Antibody[104.2] 50Tests, 100Tests, 100Tests×2 Check More
PE/Cyanine5 Anti-Mouse CD45.2 Antibody[104.2] 50Tests, 100Tests, 100Tests×2 Check More
PE/Cyanine7 Anti-Mouse CD45.2 Antibody[104.2] 50Tests, 100Tests, 100Tests×2 Check More
PE/Cyanine5.5 Anti-Mouse CD45.2 Antibody[104.2] 50Tests, 100Tests, 100Tests×2 Check More
PerCP/Cyanine5.5 Anti-Mouse CD45.2 Antibody[104.2] 50Tests, 100Tests, 100Tests×2 Check More
Elab Fluor® 488 Anti-Mouse CD45.2 Antibody[104.2] 50Tests, 100Tests, 100Tests×2 Check More
Elab Fluor® 647 Anti-Mouse CD45.2 Antibody[104.2] 50Tests, 100Tests, 100Tests×2 Check More
FITC Anti-Mouse CD45.2 Antibody[104.2] 25μg, 100μg Check More
PE Anti-Mouse CD45.2 Antibody[104.2] 25μg, 100μg Check More
APC Anti-Mouse CD45.2 Antibody[104.2] 25μg, 100μg Check More
PE/Cyanine5 Anti-Mouse CD45.2 Antibody[104.2] 25μg, 100μg Check More
PE/Cyanine7 Anti-Mouse CD45.2 Antibody[104.2] 25μg, 100μg Check More
PE/Cyanine5.5 Anti-Mouse CD45.2 Antibody[104.2] 25μg, 100μg Check More
PerCP/Cyanine5.5 Anti-Mouse CD45.2 Antibody[104.2] 25μg, 100μg Check More
Elab Fluor® 488 Anti-Mouse CD45.2 Antibody[104.2] 25μg, 100μg Check More
Elab Fluor® 647 Anti-Mouse CD45.2 Antibody[104.2] 25μg, 100μg Check More

Citations

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