Alternate Names	T-cell surface glycoprotein CD4,CD4,T-cell surface antigen T4/Leu-3,CD4
Swissprot	P06332
References	1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Bierer BE, et al. 1989. Annu. Rev. Immunol. 7:579. 3. Janeway CA. 1992. Annu. Rev. Immunol. 10:645.

Product Details

Form	Liquid
Clone No.	GK1.5(See Other Available Formats)
Host	Rat
Isotype	Rat IgG2b, κ
Isotype Control	PE Rat IgG2b, κ Isotype Control[LTF-2] [Product E-AB-F09843D]
Reactivity	Mouse
Application	FCM (See the Application Description)
Storage Buffer	Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use	Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/10⁶ cells in 100 μL volume].
Shipping	Biological ice pack at 4℃
Stability & Storage	Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Conjugation	PE
	PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 585/42 nm bandpass filter).

How to select the appropriate detection channel through the spectrogram?

Background

CD4 is a 55 kD protein also known as L3T4 or T4. It is a member of the Ig superfamily, primarily expressed on most thymocytes, a subset of T cells, and weakly on macrophages and dendritic cells. It acts as a coreceptor with the TCR during T cell activation and thymic differentiation by binding MHC class II and associating with the protein tyrosin kinase, lck.

Protocols & Troubleshootings

Related Protocols	Staining Cell Surface Targets for Flow Cytometry
Troubleshooting	Troubleshooting for Flow Cytometry

Alternative Formats

Product Name	Sizes	Price
AF/LE Purified Anti-Mouse CD4 Antibody[GK1.5]	50μg, 500μg, 1mg	Check More
Purified Anti-Mouse CD4 Antibody[GK1.5]	25μg, 100μg	Check More
Biotin Anti-Mouse CD4 Antibody[GK1.5]	25μg, 100μg	Check More
FITC Anti-Mouse CD4 Antibody[GK1.5]	50Tests, 100Tests, 100Tests×2	Check More
PE Anti-Mouse CD4 Antibody[GK1.5]	50Tests, 100Tests, 100Tests×2	Check More
APC Anti-Mouse CD4 Antibody[GK1.5]	50Tests, 100Tests, 100Tests×2	Check More
PerCP Anti-Mouse CD4 Antibody[GK1.5]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5 Anti-Mouse CD4 Antibody[GK1.5]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine7 Anti-Mouse CD4 Antibody[GK1.5]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5.5 Anti-Mouse CD4 Antibody[GK1.5]	50Tests, 100Tests, 100Tests×2	Check More
PerCP/Cyanine5.5 Anti-Mouse CD4 Antibody[GK1.5]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 488 Anti-Mouse CD4 Antibody[GK1.5]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 647 Anti-Mouse CD4 Antibody[GK1.5]	50Tests, 100Tests, 100Tests×2	Check More
PE/Elab Fluor^® 594 Anti-Mouse CD4 Antibody[GK1.5]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® Violet 450 Anti-Mouse CD4 Antibody[GK1.5]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® Red 780 Anti-Mouse CD4 Antibody[GK1.5]	50Tests, 100Tests, 100Tests×2	Check More
FITC Anti-Mouse CD4 Antibody[GK1.5]	25μg, 100μg	Check More
APC Anti-Mouse CD4 Antibody[GK1.5]	25μg, 100μg	Check More
PerCP Anti-Mouse CD4 Antibody[GK1.5]	25μg, 100μg	Check More
PE/Cyanine5 Anti-Mouse CD4 Antibody[GK1.5]	25μg, 100μg	Check More
PE/Cyanine7 Anti-Mouse CD4 Antibody[GK1.5]	25μg, 100μg	Check More
PE/Cyanine5.5 Anti-Mouse CD4 Antibody[GK1.5]	25μg, 100μg	Check More
PerCP/Cyanine5.5 Anti-Mouse CD4 Antibody[GK1.5]	25μg, 100μg	Check More
Elab Fluor^® 488 Anti-Mouse CD4 Antibody[GK1.5]	25μg, 100μg	Check More
Elab Fluor^® 647 Anti-Mouse CD4 Antibody[GK1.5]	25μg, 100μg	Check More
PE/Elab Fluor^® 594 Anti-Mouse CD4 Antibody[GK1.5]	25μg, 100μg	Check More
Elab Fluor^® Violet 450 Anti-Mouse CD4 Antibody[GK1.5]	25μg, 100μg	Check More
Elab Fluor^® Red 780 Anti-Mouse CD4 Antibody[GK1.5]	25μg, 100μg	Check More

Citations

Marine Drugs (2022) IF: 5.118
In Vitro and In Vivo Dendritic Cell Immune Stimulation Effect of Low Molecular Weight Fucoidan from New Zealand Undaria pinnatifida

DOI: 10.3390/md20030197

PMID: 35323496
Sample: Dendritic cells (DCs)
Journal of Integrative Medicine-JIM (2022) IF: 3.034
Moxibustion regulates T-regulatory/T-helper 17 cell balance by modulating the microRNA-221/suppressor of cytokine signaling 3 axis in a mouse model of rheumatoid arthritis

DOI: 10.1016/j.joim.2022.06.002

PMID: 35729047
Sample: splenocytes

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