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Current Position:Home>>Antibodies>>Flow Cytometry Antibodies>> PE Anti-Mouse CD80 Antibody[16-10A1] E-AB-F0992D

PE Anti-Mouse CD80 Antibody[16-10A1] E-AB-F0992D

Uniprot : Q00609

  • Cat.No.:E-AB-F0992D

  • Applications: FCM
  • Isotype: Armenian Hamster IgG
  • Host: Armenian Hamster
Applications:

Manual MSDS

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Antigen Details

Alternate Names T-lymphocyte activation antigen CD80,Cd80,Activation B7-1 antigen,B7
Swissprot Q00609
References
1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Linsley PS, et al. 1991. J. Exp. Med. 174:561.
3. Salomon B, et al. 2001. Annu. Rev. Immunol. 19:225.

Product Details

Form Liquid
Clone No. 16-10A1(See Other Available Formats)
Host Armenian Hamster
Isotype Armenian Hamster IgG
Isotype Control PE Armenian Hamster IgG Isotype Control[PIP] [Product E-AB-F09852D]
Reactivity Mouse
Application FCM (See the Application Description)
Storage Buffer Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping Biological ice pack at 4℃
Stability & Storage Keep as concentrated solution.
Store at 2~8°C and protected from prolonged exposure to light. Do not freeze.
Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.
Conjugation PE
PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 585/42 nm bandpass filter).


How to select the appropriate detection channel through the spectrogram?

Background

CD80 is a 60 kD highly glycosylated protein. It is a member of the Ig superfamily and is also known as B7-1, B7, and Ly-53. CD80 is constitutively expressed on dendritic cells and monocytes/macrophages, and inducibly expressed on activated B and T cells. The ligation of CD28 on T cells with CD80 and CD86 (B7-2) on antigen presenting cells (such as dendritic cells, macrophages, and B cells) elicits co-stimulation of T cells resulting in enhanced cell activation, proliferation, and cytokine production. CD80 appears to be expressed later in the immune response than CD86. CD80 can also bind to CD152, also known as CTLA-4, to deliver an inhibitory signal to T cells.

Protocols & Troubleshootings

Related Protocols Staining Cell Surface Targets for Flow Cytometry
Troubleshooting

Troubleshooting for Flow Cytometry

Alternative Formats

Product Name Sizes Price
AF/LE Purified Anti-Mouse CD80 Antibody[16-10A1] E-AB-F09920 50μg, 500μg, 1mg Check More
Purified Anti-Mouse CD80 Antibody[16-10A1] E-AB-F0992A 25μg, 100μg Check More
Biotin Anti-Mouse CD80 Antibody[16-10A1] E-AB-F0992B 25μg, 100μg Check More
FITC Anti-Mouse CD80 Antibody[16-10A1] E-AB-F0992C 50Tests, 100Tests, 100Tests×2 Check More
APC Anti-Mouse CD80 Antibody[16-10A1] E-AB-F0992E 50Tests, 100Tests, 100Tests×2 Check More
PE/Cyanine5 Anti-Mouse CD80 Antibody[16-10A1] E-AB-F0992G 50Tests, 100Tests, 100Tests×2 Check More
PE/Cyanine7 Anti-Mouse CD80 Antibody[16-10A1] E-AB-F0992H 50Tests, 100Tests, 100Tests×2 Check More
PE/Cyanine5.5 Anti-Mouse CD80 Antibody[16-10A1] E-AB-F0992I 50Tests, 100Tests, 100Tests×2 Check More
PerCP/Cyanine5.5 Anti-Mouse CD80 Antibody[16-10A1] E-AB-F0992J 50Tests, 100Tests, 100Tests×2 Check More
PE/Elab Fluor® 594 Anti-Mouse CD80 Antibody[16-10A1] 50Tests, 100Tests, 100Tests×2 Check More
Elab Fluor® Violet 450 Anti-Mouse CD80 Antibody[16-10A1] 50Tests, 100Tests, 100Tests×2 Check More
Elab Fluor® Red 780 Anti-Mouse CD80 Antibody[16-10A1] 50Tests, 100Tests, 100Tests×2 Check More
FITC Anti-Mouse CD80 Antibody[16-10A1] E-AB-F0992UC 25μg, 100μg Check More
PE Anti-Mouse CD80 Antibody[16-10A1] E-AB-F0992UD 25μg, 100μg Check More
APC Anti-Mouse CD80 Antibody[16-10A1] E-AB-F0992UE 25μg, 100μg Check More
PE/Cyanine5 Anti-Mouse CD80 Antibody[16-10A1] E-AB-F0992UG 25μg, 100μg Check More
PE/Cyanine7 Anti-Mouse CD80 Antibody[16-10A1] E-AB-F0992UH 25μg, 100μg Check More
PE/Cyanine5.5 Anti-Mouse CD80 Antibody[16-10A1] E-AB-F0992UI 25μg, 100μg Check More
PerCP/Cyanine5.5 Anti-Mouse CD80 Antibody[16-10A1] E-AB-F0992UJ 25μg, 100μg Check More
PE/Elab Fluor® 594 Anti-Mouse CD80 Antibody[16-10A1] 25μg, 100μg Check More
Elab Fluor® Violet 450 Anti-Mouse CD80 Antibody[16-10A1] 25μg, 100μg Check More
Elab Fluor® Red 780 Anti-Mouse CD80 Antibody[16-10A1] 25μg, 100μg Check More

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