Current Position:Home>>Antibodies>>Flow Cytometry Antibodies>> PE Anti-Mouse Ly6G Antibody[1A8] E-AB-F1108D
Cat.No.:E-AB-F1108D
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Alternate Names | Lymphocyte antigen 6G,Ly-6G,Ly-6G.1,Ly6g |
Swissprot | P35461 |
References |
1.Fleming TJ, Fleming ML, Malek TR. Selective expression of Ly-6G on myeloid lineage cells in mouse bone marrow. J Immunol. 1993; 151(5):2399-2408.
2.Hestdal K, Ruscetti FW, Ihle JN, et al. Characterization and regulation of RB6-8C5 antigen expression on murine bone marrow cells. J Immunol. 1991;147(1):22-28. 3.Jutila MA, Kroese FG, Jutila KL, et al. Ly-6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon-gamma. Eur J Immunol. 1988; 18(11):1819-1826. 4.Lagasse E, Weissman IL. Flow cytometric identification of murine neutrophils and monocytes. J Immunol Methods. 1996; 197(1-2):139-150. |
Form | Liquid |
Clone No. | 1A8(See Other Available Formats) |
Host | Rat |
Isotype | Rat IgG2a, κ |
Isotype Control | PE Rat IgG2a, κ Isotype Control[2A3] [Product E-AB-F09832D] |
Reactivity | Mouse |
Application | FCM (See the Application Description) |
Storage Buffer | Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant. |
Recommended Use | Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. |
Shipping | Biological ice pack at 4℃ |
Stability & Storage | Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase. |
Conjugation | PE |
PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 585/42 nm bandpass filter). | |
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How to select the appropriate detection channel through the spectrogram?
Lymphocyte antigen 6 complex, locus G (Ly-6G), a 21-25 kD GPI-anchored protein, is expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes. |
Related Protocols | Staining Cell Surface Targets for Flow Cytometry |
Troubleshooting |
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Sample: Cell sample |