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PE Anti-Mouse MHC II (I-A/I-E) Antibody[M5/114]

  • Cat.No.:E-AB-F0990D

  • Applications: FCM
  • Isotype: Rat IgG2b, κ
  • Host: Rat

To Purchase E-AB-F0990D

Size:
  • 50Tests
  • 100Tests
  • 100Tests×2
Price: $28
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Antigen Details

Alternate Names H2-Ab1/Eb1,Major histocompatibility protein class II beta chain,MHC class II H2-IA-beta-psi,I-E beta MHC class II,MHC class II
Swissprot P14483,O78196
References
1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.

Product Details

Form Liquid
Clone No. M5/114(See Other Available Formats)
Host Rat
Isotype Rat IgG2b, κ
Isotype Control PE Rat IgG2b, κ Isotype Control[LTF-2] [Product E-AB-F09842D]
Reactivity Mouse
Application FCM (See the Application Description)
Storage Buffer Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping Biological ice pack at 4℃
Stability & Storage Keep as concentrated solution.
Store at 2~8°C and protected from prolonged exposure to light. Do not freeze.
Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.
Conjugation PE
PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 585/42 nm bandpass filter).


How to select the appropriate detection channel through the spectrogram?

Background

These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2b,d,q,r bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins.

Protocols & Troubleshootings

Related Protocols Staining Cell Surface Targets for Flow Cytometry
Troubleshooting

Troubleshooting for Flow Cytometry

Alternative Formats

Product Name Sizes Price
AF/LE Purified Anti-Mouse MHC II (I-A/I-E) Antibody[M5/114] 50μg, 500μg, 1mg Check More
Purified Anti-Mouse MHC II (I-A/I-E) Antibody[M5/114] 25μg, 100μg Check More
Biotin Anti-Mouse MHC II (I-A/I-E) Antibody[M5/114] 25μg, 100μg Check More
FITC Anti-Mouse MHC II (I-A/I-E) Antibody[M5/114] 50Tests, 100Tests, 100Tests×2 Check More
APC Anti-Mouse MHC II (I-A/I-E) Antibody[M5/114] 50Tests, 100Tests, 100Tests×2 Check More
PerCP Anti-Mouse MHC II (I-A/I-E) Antibody[M5/114] 50Tests, 100Tests, 100Tests×2 Check More
PE/Cyanine5 Anti-Mouse MHC II (I-A/I-E) Antibody[M5/114] 50Tests, 100Tests, 100Tests×2 Check More
PE/Cyanine7 Anti-Mouse MHC II (I-A/I-E) Antibody[M5/114] 50Tests, 100Tests, 100Tests×2 Check More
PE/Cyanine5.5 Anti-Mouse MHC II (I-A/I-E) Antibody[M5/114] 50Tests, 100Tests, 100Tests×2 Check More
PerCP/Cyanine5.5 Anti-Mouse MHC II (I-A/I-E) Antibody[M5/114] 50Tests, 100Tests, 100Tests×2 Check More
Elab Fluor® 488 Anti-Mouse MHC II (I-A/I-E) Antibody[M5/114] 50Tests, 100Tests, 100Tests×2 Check More
Elab Fluor® 647 Anti-Mouse MHC II (I-A/I-E) Antibody[M5/114] 50Tests, 100Tests, 100Tests×2 Check More
Elab Fluor® Violet 450 Anti-Mouse MHC II (I-A/I-E) Antibody[M5/114] 50Tests, 100Tests, 100Tests×2 Check More
FITC Anti-Mouse MHC II (I-A/I-E) Antibody[M5/114] 25μg, 100μg Check More
PE Anti-Mouse MHC II (I-A/I-E) Antibody[M5/114] 25μg, 100μg Check More
APC Anti-Mouse MHC II (I-A/I-E) Antibody[M5/114] 25μg, 100μg Check More
PerCP Anti-Mouse MHC II (I-A/I-E) Antibody[M5/114] 25μg, 100μg Check More
PE/Cyanine5 Anti-Mouse MHC II (I-A/I-E) Antibody[M5/114] 25μg, 100μg Check More
PE/Cyanine7 Anti-Mouse MHC II (I-A/I-E) Antibody[M5/114] 25μg, 100μg Check More
PE/Cyanine5.5 Anti-Mouse MHC II (I-A/I-E) Antibody[M5/114] 25μg, 100μg Check More
PerCP/Cyanine5.5 Anti-Mouse MHC II (I-A/I-E) Antibody[M5/114] 25μg, 100μg Check More
Elab Fluor® 488 Anti-Mouse MHC II (I-A/I-E) Antibody[M5/114] 25μg, 100μg Check More
Elab Fluor® 647 Anti-Mouse MHC II (I-A/I-E) Antibody[M5/114] 25μg, 100μg Check More
Elab Fluor® Violet 450 Anti-Mouse MHC II (I-A/I-E) Antibody[M5/114] 25μg, 100μg Check More

Citations

  1. JOURNAL OF ETHNOPHARMACOLOGY (2021) IF: 4.36
    Effect of Si-Miao-Yong-An decoction on the differentiation of monocytes, macrophages, and regulatory T cells in ApoE-/- mice

    DOI: 10.1016/j.jep.2021.114178

    Sample: Cell Culture Supernatant
  2. INTERNATIONAL IMMUNOPHARMACOLOGY (2023) IF: 5.714
    Dioscin modulates macrophages polarization and MDSCs differentiation to inhibit tumorigenesis of colitis-associated colorectal cancer

    DOI: 10.1016/j.intimp.2023.109839

    Sample: Blood,Spleen,Myeloid-Derived Suppressor Cells (Mds

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