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Cat.No.:E-AB-F1225D
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Alternate Names | Pgp-1, H-CAM, CD44s,CD44H |
Swissprot | P26051 |
References |
1. Arch R, et al. 1992. Science 257 (5070):682
2. Mitnacht R, et al. 1995. Eur. J. Immunol. 25(2):328 3. Zheng Z, et al. 1995. J Cell Bio. 130(2):485 4. Westermann J, et al. 1994. Eur. J. Immunol. 24(10):2312 |
Form | Liquid |
Clone No. | OX-49 |
Host | Mouse |
Isotype | Mouse IgG2a, κ |
Isotype Control | PE Mouse IgG2a, κ Isotype Control[C1.18.4] [Product E-AB-F09802D] |
Reactivity | Rat |
Application | FCM |
Storage Buffer | Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant. |
Recommended Use | Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. |
Shipping | Biological ice pack at 4℃ |
Stability & Storage | Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase. |
Conjugation | PE |
PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 585/42 nm bandpass filter). | |
How to select the appropriate detection channel through the spectrogram?
CD44 is an 80-95 kD cell surface glycoprotein. It is expressed on all leukocytes, endothelial cells, hepatocytes, and mesenchymal cells. It is up-regulated when T cells and B cells are activated. It was reported that CD44 is a valuable marker for memory T cells. CD44 is an adhesion molecule involved in leukocyte adhesion and homing to lymphoid organs. The OX-49 antibody reacts with CD44H (known as CD44s) expressed on most leukocytes, except for a subset of B lymphocytes. The epitope recognized by OX-49 antibody has been mapped to a region on both the standard, CD44s, and the splice variant, CD44v, isoforms of CD44. However it was reported that OX-49 antibody cannot detect the CD44V isoform, possibly due to conformational changes in the epitope. |
Related Protocols | Staining Cell Surface Targets for Flow Cytometry |
Troubleshooting |
Product Name | Sizes | Price |
FITC Anti-Rat CD44H Antibody[OX-49] | 50Tests, 100Tests, 100Tests×2 | Check More |
APC Anti-Rat CD44H Antibody[OX-49] | 50Tests, 100Tests, 100Tests×2 | Check More |
PE/Cyanine5 Anti-Rat CD44H Antibody[OX-49] | 50Tests, 100Tests, 100Tests×2 | Check More |
PE/Cyanine7 Anti-Rat CD44H Antibody[OX-49] | 50Tests, 100Tests, 100Tests×2 | Check More |
PerCP/Cyanine5.5 Anti-Rat CD44H Antibody[OX-49] | 50Tests, 100Tests, 100Tests×2 | Check More |
FITC Anti-Rat CD44H Antibody[OX-49] | 25μg, 100μg | Check More |
PE Anti-Rat CD44H Antibody[OX-49] | 25μg, 100μg | Check More |
APC Anti-Rat CD44H Antibody[OX-49] | 25μg, 100μg | Check More |
PE/Cyanine5 Anti-Rat CD44H Antibody[OX-49] | 25μg, 100μg | Check More |
PE/Cyanine7 Anti-Rat CD44H Antibody[OX-49] | 25μg, 100μg | Check More |
PerCP/Cyanine5.5 Anti-Rat CD44H Antibody[OX-49] | 25μg, 100μg | Check More |