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PE/Cyanine5.5 Anti-Human CD27 Antibody[O323]

Uniprot : P26842
  • Cat.No.:E-AB-F1140I

  • Applications: FCM
  • Isotype: Mouse IgG1, κ
  • Host: Mouse

To Purchase E-AB-F1140I

Size:
  • 20Tests
  • 100Tests
  • 100Tests×2
Price: $52
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Antigen Details

Alternate Names CD27L receptor,T-cell activation antigen CD27,T14,S152,TNFRSF7
Swissprot P26842
References
1. Hintzen R, et al. 1994. Immunol. Today 15:307.
2. Agematsu K, et al. 1995. J. Immunol. 154:3627.

Product Details

Form Liquid
Clone No. O323(See Other Available Formats)
Host Mouse
Isotype Mouse IgG1, κ
Isotype Control PE/Cyanine5.5 Mouse IgG1, κ Isotype Control[MOPC-21] [Product E-AB-F09792I]
Reactivity Human
Application FCM (See the Application Description)
Storage Buffer Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping Biological ice pack at 4℃
Stability & Storage Keep as concentrated solution.
Store at 2~8°C and protected from prolonged exposure to light. Do not freeze.
Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.
Conjugation PE/Cyanine5.5
PE/Cyanine5.5 is designed to be excited by the Blue (488 nm), Green (532 nm) and yellow-green (561 nm) lasers and detected using an optical filter centered near 690 nm (e.g., a 690/50 nm bandpass filter).


How to select the appropriate detection channel through the spectrogram?

Background

CD27 is a 50-55 kD type I membrane protein also known as S152 and T14. It is a lymphocyte-specific member of the TNF-receptor superfamily. CD27 is expressed on medullary thymocytes, virtually all mature T cells, some B cells, and NK cells. CD27 binds to CD70 and plays an important role in costimulation of T cell activation, and regulation of B cell differentiation and proliferation. The cytoplasmic domains of CD27 have also been shown to interact with TRAF2 and TRAF5 to elicit NF-κB and SAPK/JNK activation.

Protocols & Troubleshootings

Related Protocols Staining Cell Surface Targets for Flow Cytometry
Troubleshooting

Troubleshooting for Flow Cytometry

Alternative Formats

Product Name Sizes Price
FITC Anti-Human CD27 Antibody[O323] 20Tests, 100Tests, 100Tests×2 Check More
PE Anti-Human CD27 Antibody[O323] 20Tests, 100Tests, 100Tests×2 Check More
APC Anti-Human CD27 Antibody[O323] 20Tests, 100Tests, 100Tests×2 Check More
PE/Cyanine5 Anti-Human CD27 Antibody[O323] 20Tests, 100Tests, 100Tests×2 Check More
PE/Cyanine7 Anti-Human CD27 Antibody[O323] 20Tests, 100Tests, 100Tests×2 Check More
PerCP/Cyanine5.5 Anti-Human CD27 Antibody[O323] 20Tests, 100Tests, 100Tests×2 Check More
Elab Fluor® 488 Anti-Human CD27 Antibody[O323] 20Tests, 100Tests, 100Tests×2 Check More
Elab Fluor® 647 Anti-Human CD27 Antibody[O323] 20Tests, 100Tests, 100Tests×2 Check More
Elab Fluor® Red 780 Anti-Human CD27 Antibody[O323] 20Tests, 100Tests, 100Tests×2 Check More

Citations

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