Current Position:Home>>Antibodies>>Flow Cytometry Antibodies>> PE/Cyanine5.5 Anti-Mouse CD11c Antibody[N418]
Cat.No.:E-AB-F0991UI
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Alternate Names | Integrin alpha-X,Itgax,CD11 antigen-like family member C,Leukocyte adhesion receptor p150+95,CD11c |
Swissprot | Q9QXH4 |
References |
1. Barclay A, et al. 1997. The Leukocyte Antigen Facts Book Academic Press.
2. Springer TA. 1994. Cell 76:301. 3. Lopez-Rodriguez C, et al. 1996. J. Immunol. 156:3780. |
Form | Liquid |
Clone No. | N418(See Other Available Formats) |
Host | Armenian Hamster |
Isotype | Armenian Hamster IgG |
Isotype Control | PE/Cyanine5.5 Armenian Hamster IgG Isotype Control[PIP] [Product E-AB-F09853I] |
Reactivity | Mouse |
Application | FCM (See the Application Description) |
Storage Buffer | Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant. |
Recommended Use | Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/106 cells in 100 μL volume]. |
Shipping | Biological ice pack at 4℃ |
Stability & Storage | Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase. |
Conjugation | PE/Cyanine5.5 |
PE/Cyanine5.5 is designed to be excited by the Blue (488 nm), Green (532 nm) and yellow-green (561 nm) lasers and detected using an optical filter centered near 690 nm (e.g., a 690/50 nm bandpass filter). | |
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How to select the appropriate detection channel through the spectrogram?
CD11c is a 150 kD glycoprotein also known as αX integrin, CR4, and p150. CD11c forms a αXβ2 heterodimer with β2 integrin (CD18). It is primarily expressed on dendritic cells, NK cells, a subset of intestinal intraepithelial lymphocytes (IEL), and some activated T cells. The αXβ2 integrin plays an important role in cell-cell contact by binding its ligands: iC3b, fibrinogen and CD54. |
Related Protocols | Staining Cell Surface Targets for Flow Cytometry |
Troubleshooting |
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