Current Position:Home>>Antibodies>>Flow Cytometry Antibodies>> PE/Cyanine5.5 Anti-Mouse CD45 Antibody[30-F11] E-AB-F1136UI

PE/Cyanine5.5 Anti-Mouse CD45 Antibody[30-F11] E-AB-F1136UI

Uniprot : P06800

Cat.No.:E-AB-F1136UI
Applications: FCM
Isotype: Rat IgG2b, κ
Host: Rat

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Antigen Details

Alternate Names	Receptor-type tyrosine-protein phosphatase C,Ptprc,L-CA,Ly-5,T200,CD45
Swissprot	P06800
References	1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85. 3. Kishihara K, et al. 1993. Cell 74:143. 4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Product Details

Form	Liquid
Clone No.	30-F11(See Other Available Formats)
Host	Rat
Isotype	Rat IgG2b, κ
Isotype Control	PE/Cyanine5.5 Rat IgG2b, κ Isotype Control[LTF-2] [Product E-AB-F09843I]
Reactivity	Mouse
Application	FCM (See the Application Description)
Storage Buffer	Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use	Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/10⁶ cells in 100 μL volume].
Shipping	Biological ice pack at 4℃
Stability & Storage	Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Conjugation	PE/Cyanine5.5
	PE/Cyanine5.5 is designed to be excited by the Blue (488 nm), Green (532 nm) and yellow-green (561 nm) lasers and detected using an optical filter centered near 690 nm (e.g., a 690/50 nm bandpass filter).

How to select the appropriate detection channel through the spectrogram?

Background

CD45 is a 180-240 kD glycoprotein also known as the leukocyte common antigen (LCA), T200, or Ly-5. It is a member of the protein tyrosine phosphatase (PTP) family, expressed on all hematopoietic cells except mature erythrocytes and platelets. There are different isoforms of CD45 that arise from alternative splicing of exons 4, 5, and 6, which encode A, B, and C determinants, respectively. CD45 plays a key role in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation state of the cell as well as cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Protocols & Troubleshootings

Related Protocols	Staining Cell Surface Targets for Flow Cytometry
Troubleshooting	Troubleshooting for Flow Cytometry

Alternative Formats

Product Name	Sizes	Price
FITC Anti-Mouse CD45 Antibody[30-F11] E-AB-F1136C	50Tests, 100Tests, 100Tests×2	Check More
PE Anti-Mouse CD45 Antibody[30-F11] E-AB-F1136D	50Tests, 100Tests, 100Tests×2	Check More
APC Anti-Mouse CD45 Antibody[30-F11] E-AB-F1136E	50Tests, 100Tests, 100Tests×2	Check More
PerCP Anti-Mouse CD45 Antibody[30-F11]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5 Anti-Mouse CD45 Antibody[30-F11] E-AB-F1136G	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine7 Anti-Mouse CD45 Antibody[30-F11] E-AB-F1136H	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5.5 Anti-Mouse CD45 Antibody[30-F11] E-AB-F1136I	50Tests, 100Tests, 100Tests×2	Check More
PerCP/Cyanine5.5 Anti-Mouse CD45 Antibody[30-F11] E-AB-F1136J	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 488 Anti-Mouse CD45 Antibody[30-F11]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 647 Anti-Mouse CD45 Antibody[30-F11]	50Tests, 100Tests, 100Tests×2	Check More
PE/Elab Fluor^® 594 Anti-Mouse CD45 Antibody[30-F11]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® Violet 450 Anti-Mouse CD45 Antibody[30-F11]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® Red 780 Anti-Mouse CD45 Antibody[30-F11]	50Tests, 100Tests, 100Tests×2	Check More
FITC Anti-Mouse CD45 Antibody[30-F11] E-AB-F1136UC	25μg, 100μg	Check More
PE Anti-Mouse CD45 Antibody[30-F11] E-AB-F1136UD	25μg, 100μg	Check More
APC Anti-Mouse CD45 Antibody[30-F11] E-AB-F1136UE	25μg, 100μg	Check More
PerCP Anti-Mouse CD45 Antibody[30-F11]	25μg, 100μg	Check More
PE/Cyanine5 Anti-Mouse CD45 Antibody[30-F11] E-AB-F1136UG	25μg, 100μg	Check More
PE/Cyanine7 Anti-Mouse CD45 Antibody[30-F11] E-AB-F1136UH	25μg, 100μg	Check More
PerCP/Cyanine5.5 Anti-Mouse CD45 Antibody[30-F11] E-AB-F1136UJ	25μg, 100μg	Check More
Elab Fluor^® 488 Anti-Mouse CD45 Antibody[30-F11]	25μg, 100μg	Check More
Elab Fluor^® 647 Anti-Mouse CD45 Antibody[30-F11]	25μg, 100μg	Check More
PE/Elab Fluor^® 594 Anti-Mouse CD45 Antibody[30-F11]	25μg, 100μg	Check More
Elab Fluor^® Violet 450 Anti-Mouse CD45 Antibody[30-F11]	25μg, 100μg	Check More
Elab Fluor^® Red 780 Anti-Mouse CD45 Antibody[30-F11]	25μg, 100μg	Check More

Citations

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