Current Position:Home>>Antibodies>>Flow Cytometry Antibodies>> PE/Cyanine5 Anti-Human CD123 Antibody[6H6] E-AB-F1117G
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|Alternate Names||IL-3Rα, IL-3 Receptor alpha|
1. Miyajima A, et al. 1993. Blood 82:1960.
|Clone No.||6H6(See Other Available Formats)|
|Isotype||Mouse IgG1, κ|
|Isotype Control||[Product E-AB-F09792G]|
|Application||FCM (See the Application Description)|
|Storage Buffer||Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.|
|Recommended Use||Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.|
|Shipping||Biological ice pack at 4℃|
|Stability & Storage||Keep as concentrated solution.
Store at 2~8°C and protected from prolonged exposure to light. Do not freeze.
Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.
|PE/Cyanine5 is designed to be excited by the Blue (488 nm), Green (532 nm) and yellow-green (561 nm) lasers and detected using an optical filter centered near 670 nm (e.g., a 690/50 nm bandpass filter).|
How to select the appropriate detection channel through the spectrogram?
|CD123 is the 70 kD transmembrane α chain of the IL-3 receptor. Alone, CD123 binds IL-3 with low affinity; when CD123 associates with CDw131 (common β chain), it binds IL-3 with high affinity. CD123 does not transduce intracellular signals upon binding IL-3 and requires the β chain for this function. CD123 is expressed by myeloid precursors, macrophages, dendritic cells, mast cells, basophils, megakaryocytes, and some B cells.|
|Related Protocols||Staining Cell Surface Targets for Flow Cytometry|
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