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PE/Cyanine5 Anti-Human CD4 Antibody[RPA-T4]

Uniprot : P01730
  • Cat.No.:E-AB-F1109G

  • Applications: FCM
  • Isotype: Mouse IgG1, κ
  • Host: Mouse

To Purchase E-AB-F1109G

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Antigen Details

Alternate Names T-cell surface glycoprotein CD4,CD4,T-cell surface antigen T4/Leu-3,CD4
Swissprot P01730
References
1. Zhu J, et al. 2010. Annu Rev. Immunol. 28:445.
2. Vignali DA. 2010. J. Immunol. 184:5933.
3. Zhou L, et al. 2009. Immunity 30:646.
4. Singer A, et al. 2008. Nat. Rev. Immunol. 8:788.
5. Zhu J and Paul WE. 2008. Blood 112:1557.

Product Details

Form Liquid
Clone No. RPA-T4(See Other Available Formats)
Host Mouse
Isotype Mouse IgG1, κ
Isotype Control [Product E-AB-F09792G]
Reactivity Human
Application FCM (See the Application Description)
Storage Buffer Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping Biological ice pack at 4℃
Stability & Storage Keep as concentrated solution.
Store at 2~8°C and protected from prolonged exposure to light. Do not freeze.
Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.
Conjugation PE/Cyanine5
PE/Cyanine5 is designed to be excited by the Blue (488 nm), Green (532 nm) and yellow-green (561 nm) lasers and detected using an optical filter centered near 670 nm (e.g., a 690/50 nm bandpass filter).


How to select the appropriate detection channel through the spectrogram?

Background

CD4, also known as T4/Leu-3, is a 55 kD single-chain type I transmembrane glycoprotein and member of the immunoglobulin superfamily. It is expressed on most thymocytes, helper T cells, type II NKT cells, and monocytes/macrophages. CD4 is part of the TCR/CD3 complex, binds to β2 domain from the MHC class II molecule, and participates in TCR signal transduction. CD4 is the receptor of IL-16 and is a coreceptor for the human immunodeficiency virus (HIV) and human herpes virus 7 (HHV-7).

Protocols & Troubleshootings

Related Protocols Staining Cell Surface Targets for Flow Cytometry
Troubleshooting

Troubleshooting for Flow Cytometry

Citations

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