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PE/Cyanine5 Anti-Mouse CD335/NKp46 Antibody[29A1.4]

Uniprot : Q8C567
  • Cat.No.:E-AB-F1182UG

  • Applications: FCM
  • Isotype: Rat IgG2a, κ
  • Host: Rat

To Purchase E-AB-F1182UG

Size:
  • 25μg
  • 100μg
Price: $115
Qty:

Antigen Details

Alternate Names Ly94,mAR-1,NK-p46,mNKp46,Lymphocyte antigen 94
Swissprot Q8C567
References
1. Walzer T, et al. 2007. P. Natl. Acad. Sci. USA 104:3384. (FC, Activ)
2. Walzer T, et al. 2007. Nat. Immunol. 8:1337. (FC, Activ)
3. Guerriero JL, et al. 2011. J. Immunol. 186:3517. (IHC)

Product Details

Form Liquid
Clone No. 29A1.4(See Other Available Formats)
Host Rat
Isotype Rat IgG2a, κ
Isotype Control PE/Cyanine5 Rat IgG2a, κ Isotype Control[2A3] [Product E-AB-F09833G]
Reactivity Mouse
Application FCM (See the Application Description)
Storage Buffer Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/106 cells in 100 μL volume].
Shipping Biological ice pack at 4℃
Stability & Storage Keep as concentrated solution.
Store at 2~8°C and protected from prolonged exposure to light. Do not freeze.
Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.
Conjugation PE/Cyanine5
PE/Cyanine5 is designed to be excited by the Blue (488 nm), Green (532 nm) and yellow-green (561 nm) lasers and detected using an optical filter centered near 670 nm (e.g., a 690/50 nm bandpass filter).


How to select the appropriate detection channel through the spectrogram?

Background

CD335, also known as NKp46, is a single-pass type I membrane protein of 46 kD. It belongs to the natural cytotoxicity receptor (NCR) family and contains two Ig-like (immunoglobulin-like) domains. It's expression is restricted to NK cells and a subset of NKT cells; it's not expressed in CD1d-restricted NKT cells. CD335 is a receptor for viral hemagglutinins and heparan sulfate proteoglycans and is involved in NK cell activation.

Protocols & Troubleshootings

Related Protocols Staining Cell Surface Targets for Flow Cytometry
Troubleshooting

Troubleshooting for Flow Cytometry

Citations

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