Current Position:Home>>Antibodies>>Flow Cytometry Antibodies>> PE/Cyanine5 Anti-Mouse Ly-6G/Ly-6C (Gr-1) Antibody[RB6-8C5]
Cat.No.:E-AB-F1120G
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Alternate Names | Gr-1,Gr1,Ly-6G/Ly-6C,Ly6G/Ly6C |
Swissprot | P35461,P0CW03 |
References |
1. Fleming TJ, et al. 1993. J. Immunol. 151:2399.
2. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819. 3. Goni O, et al. 2002. Int. Immunol. 14:1125. |
Form | Liquid |
Clone No. | RB6-8C5(See Other Available Formats) |
Host | Rat |
Isotype | Rat IgG2b, κ |
Isotype Control | PE/Cyanine5 Rat IgG2b, κ Isotype Control[LTF-2] [Product E-AB-F09842G] |
Reactivity | Mouse |
Application | FCM (See the Application Description) |
Storage Buffer | Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant. |
Recommended Use | Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. |
Shipping | Biological ice pack at 4℃ |
Stability & Storage | Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase. |
Conjugation | PE/Cyanine5 |
PE/Cyanine5 is designed to be excited by the Blue (488 nm), Green (532 nm) and yellow-green (561 nm) lasers and detected using an optical filter centered near 670 nm (e.g., a 690/50 nm bandpass filter). | |
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How to select the appropriate detection channel through the spectrogram?
Gr-1 is a 21-25 kD protein also known as Ly-6G/Ly-6C. This myeloid differentiation antigen is a glycosylphosphatidylinositol (GPI)-linked protein expressed on granulocytes and macrophages. In bone marrow, the expression levels of Gr-1 directly correlate with granulocyte differentiation and maturation; Gr-1 is also transiently expressed on bone marrow cells in the monocyte lineage. Immature Myeloid Gr-1+ cells play a role in the development of antitumor immunity. |
Related Protocols | Staining Cell Surface Targets for Flow Cytometry |
Troubleshooting |
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