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PE/Cyanine7 Anti-Human CD14 Antibody[M5E2]

Uniprot : P08571
  • Cat.No.:E-AB-F1209H

  • Applications: FCM
  • Isotype: Mouse IgG2a, κ
  • Host: Mouse

To Purchase E-AB-F1209H

Size:
  • 20Tests
  • 100Tests
  • 100Tests×2
Price: $90
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Antigen Details

Alternate Names Monocyte differentiation antigen CD14,CD14,Myeloid cell-specific leucine-rich glycoprotein
Swissprot P08571
References
1. Stocks SC, et al. 1990. Biochem. J. 268:275.
2. Wright SD, et al. 1990. Science 4975:1431.

Product Details

Form Liquid
Clone No. M5E2(See Other Available Formats)
Host Mouse
Isotype Mouse IgG2a, κ
Isotype Control PE/Cyanine7 Mouse IgG2a, κ Isotype Control[C1.18.4] [Product E-AB-F09802H]
Reactivity Human
Application FCM (See the Application Description)
Storage Buffer Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping Biological ice pack at 4℃
Stability & Storage Keep as concentrated solution.
Store at 2~8°C and protected from prolonged exposure to light. Do not freeze.
Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.
Conjugation PE/Cyanine7
PE/Cyanine7 is designed to be excited by the Blue (488 nm), Green (532 nm) and yellow-green (561 nm) lasers and detected using an optical filter centered near 775 nm (e.g., a 780/60 nm bandpass filter).


How to select the appropriate detection channel through the spectrogram?

Background

CD14 is a 53-55 kD glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein also known as LPS receptor. CD14 is expressed at high levels on monocytes and macrophages, and at lower levels on granulocytes. Some dendritic cell populations such as interfollicular dendritic cells, reticular dendritic cells, and Langerhans cells have also been reported to express CD14. As a high-affinity receptor for LPS, CD14 is involved in the clearance of gram-negative pathogens, and in the upregulation of adhesion molecules and expression of cytokines in monocytes and neutrophils.

Protocols & Troubleshootings

Related Protocols Staining Cell Surface Targets for Flow Cytometry
Troubleshooting

Troubleshooting for Flow Cytometry

Alternative Formats

Product Name Sizes Price
FITC Anti-Human CD14 Antibody[M5E2] 20Tests, 100Tests, 100Tests×2 Check More
PE Anti-Human CD14 Antibody[M5E2] 20Tests, 100Tests, 100Tests×2 Check More
APC Anti-Human CD14 Antibody[M5E2] 20Tests, 100Tests, 100Tests×2 Check More
PE/Cyanine5 Anti-Human CD14 Antibody[M5E2] 20Tests, 100Tests, 100Tests×2 Check More
PE/Cyanine5.5 Anti-Human CD14 Antibody[M5E2] 20Tests, 100Tests, 100Tests×2 Check More
PerCP/Cyanine5.5 Anti-Human CD14 Antibody[M5E2] 20Tests, 100Tests, 100Tests×2 Check More
Elab Fluor® 488 Anti-Human CD14 Antibody[M5E2] 20Tests, 100Tests, 100Tests×2 Check More
PE/Elab Fluor® 594 Anti-Human CD14 Antibody[M5E2] 20Tests, 100Tests, 100Tests×2 Check More
Elab Fluor® Violet 450 Anti-Human CD14 Antibody[M5E2] 20Tests, 100Tests, 100Tests×2 Check More
Elab Fluor® Red 780 Anti-Human CD14 Antibody[M5E2] 20Tests, 100Tests, 100Tests×2 Check More

Citations

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