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PE/Cyanine7 Anti-Human CD1a Antibody[OKT-6]

Uniprot : P06126
  • Cat.No.:E-AB-F1126H

  • Applications: FCM
  • Isotype: Mouse IgG1, κ
  • Host: Mouse

To Purchase E-AB-F1126H

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Antigen Details

Alternate Names T6, R4,CD 1a,CD1A ,T-cell surface glycoprotein CD1a
Swissprot P06126
References
1. Blumberg RS, et al. 1995. Immunol. Rev. 147:5.
2. Calabi F, et al. 1991. Tissue Antigens 37:1.
3. Melian A, et al. 1996. Curr. Opin. Immunol. 8:89.

Product Details

Form Liquid
Clone No. OKT-6(See Other Available Formats)
Host Mouse
Isotype Mouse IgG1, κ
Isotype Control PE/Cyanine7 Mouse IgG1, κ Isotype Control[MOPC-21] [Product E-AB-F09792H]
Reactivity Human
Application FCM (See the Application Description)
Storage Buffer Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping Biological ice pack at 4℃
Stability & Storage Keep as concentrated solution.
Store at 2~8°C and protected from prolonged exposure to light. Do not freeze.
Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.
Conjugation PE/Cyanine7
PE/Cyanine7 is designed to be excited by the Blue (488 nm), Green (532 nm) and yellow-green (561 nm) lasers and detected using an optical filter centered near 775 nm (e.g., a 780/60 nm bandpass filter).


How to select the appropriate detection channel through the spectrogram?

Background

CD1a is a 49 kD member of the immunoglobulin superfamily also known as T6 and R4. It is a type I membrane glycoprotein with structural similarities to MHC class I and is non-covalently associated with β2-microglobulin. CD1a plays a role in non-peptide glycolipid antigen presentation to CD1-restricted T cells. It is expressed on cortical double positive and single positive thymocytes, Langerhans cells, and dendritic cells. In addition to antigen presentation, CD1a has been implicated in thymic T cell development.

Protocols & Troubleshootings

Related Protocols Staining Cell Surface Targets for Flow Cytometry
Troubleshooting

Troubleshooting for Flow Cytometry

Citations

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