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PE/Cyanine7 Anti-Human CD40 Antibody[G28.5]

  • Cat.No.:E-AB-F1214H

  • Applications: FCM
  • Isotype: Mouse IgG1, κ
  • Host: Mouse

To Purchase E-AB-F1214H

Size:
  • 20Tests
  • 100Tests
  • 100Tests×2
Price: $85
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Antigen Details

Alternate Names Bp50,CDw40,CD40L receptor,TNFRSF5
Swissprot P25942
References
1. Banchereau J, et al. 1994. Annu. Rev. Immunol. 12:881.
2. Foy T, et al. 1996. Annu. Rev. Immunol. 14:591.

Product Details

Form Liquid
Clone No. G28.5(See Other Available Formats)
Host Mouse
Isotype Mouse IgG1, κ
Isotype Control PE/Cyanine7 Mouse IgG1, κ Isotype Control[MOPC-21] [Product E-AB-F09792H]
Reactivity Human
Application FCM (See the Application Description)
Storage Buffer Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping Biological ice pack at 4℃
Stability & Storage Keep as concentrated solution.
Store at 2~8°C and protected from prolonged exposure to light. Do not freeze.
Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.
Conjugation PE/Cyanine7
PE/Cyanine7 is designed to be excited by the Blue (488 nm), Green (532 nm) and yellow-green (561 nm) lasers and detected using an optical filter centered near 775 nm (e.g., a 780/60 nm bandpass filter).


How to select the appropriate detection channel through the spectrogram?

Background

CD40 is a 48 kD type I glycoprotein also known as BP50. It is a member of the TNFR superfamily primarily expressed on B cells, macrophages, follicular dendritic cells, endothelial cells, fibroblasts, and at low levels on plasma cells. CD40 has been reported to be involved in B cell differentiation, costimulation, isotype class-switching, and protection of B cells from apoptosis. Additionally, CD40 is important for T cell-B cell interactions. The ligand of CD40 is CD154 (CD40 ligand).

Protocols & Troubleshootings

Related Protocols Staining Cell Surface Targets for Flow Cytometry
Troubleshooting

Troubleshooting for Flow Cytometry

Alternative Formats

Product Name Sizes Price
AF/LE Purified Anti-Human CD40 Antibody[G28.5] 50μg, 500μg, 1mg Check More
Purified Anti-Human CD40 Antibody[G28.5] 25μg, 100μg Check More
FITC Anti-Human CD40 Antibody[G28.5] 20Tests, 100Tests, 100Tests×2 Check More
PE Anti-Human CD40 Antibody[G28.5] 20Tests, 100Tests, 100Tests×2 Check More
APC Anti-Human CD40 Antibody[G28.5] 20Tests, 100Tests, 100Tests×2 Check More
PE/Cyanine5 Anti-Human CD40 Antibody[G28.5] 20Tests, 100Tests, 100Tests×2 Check More
PerCP/Cyanine5.5 Anti-Human CD40 Antibody[G28.5] 20Tests, 100Tests, 100Tests×2 Check More
Elab Fluor® 488 Anti-Human CD40 Antibody[G28.5] 20Tests, 100Tests, 100Tests×2 Check More
Elab Fluor® 647 Anti-Human CD40 Antibody[G28.5] 20Tests, 100Tests, 100Tests×2 Check More
Elab Fluor® Violet 450 Anti-Human CD40 Antibody[G28.5] 20Tests, 100Tests, 100Tests×2 Check More
Elab Fluor® Red 780 Anti-Human CD40 Antibody[G28.5] 20Tests, 100Tests, 100Tests×2 Check More

Citations

  1. Biomed Research International (2022) IF: 3.411
    CD96 Downregulation Promotes the Immune Response of CD4 T Cells and Associates with Ankylosing Spondylitis

    DOI: 10.1155/2022/3946754

    PMID: 35769669

    Sample: DCs

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