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PE/Cyanine7 Anti-Human/Mouse/Rat CD47 Antibody[MIAP410]

Uniprot : Q08722,Q61735,P97829
  • Cat.No.:E-AB-F1016H

  • Applications: FCM
  • Isotype: Mouse IgG1, κ
  • Host: Mouse

To Purchase E-AB-F1016H

Size:
  • 20Tests
  • 100Tests
  • 100Tests×2
Price: $115
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Antigen Details

Alternate Names Leukocyte surface antigen CD47,Cd47,Integrin-associated protein,IAP
Swissprot Q08722,Q61735,P97829
References
1. Gresham HD, Dale BM, Potter JW, et al. J Exp Med. 2000; 191(3):515-528.
2. Jiang P, Lagenaur CF, Narayanan V. J Biol Chem. 1999; 274(2):559-562.
3. Lindberg FP, Bullard DC, Caver TE, Gresham HD, Beaudet AL, Brown EJ. Science. 1996; 274(5288):795-798.
4. Lindberg FP, Gresham HD, Schwarz E, Brown EJ. . J Cell Biol. 1993; 123(2):485-496.
5. Mi ZP, Jiang P, Weng WL, Lindberg FP, Narayanan V, Lagenaur CF. J Comp Neurol. 2000; 416(3):335-344.
6. Oldenborg PA, Zheleznyak A, Fang YF, Lagenaur CF, Gresham HD, Lindberg FP. Science. 2000; 288(5473):2051-2054.
7. Reinhold MI, Lindberg FP, Plas D, Reynolds S, Peters MG, Brown EJ. J Cell Sci. 1995 November; 108(Pt 11):3419-3425.

Product Details

Form Liquid
Clone No. MIAP410
Host Mouse
Isotype Mouse IgG1, κ
Isotype Control PE/Cyanine7 Mouse IgG1, κ Isotype Control[MOPC-21] [Product E-AB-F09792H]
Reactivity Human,Mouse,Rat
Application FCM
Storage Buffer Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping Biological ice pack at 4℃
Stability & Storage Keep as concentrated solution.
Store at 2~8°C and protected from prolonged exposure to light. Do not freeze.
Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.
Conjugation PE/Cyanine7
PE/Cyanine7 is designed to be excited by the Blue (488 nm), Green (532 nm) and yellow-green (561 nm) lasers and detected using an optical filter centered near 775 nm (e.g., a 780/60 nm bandpass filter).


How to select the appropriate detection channel through the spectrogram?

Background

CD47, also known as Integrin-Associated Protein (IAP), is a membrane protein of about 50 kD with an IgV-like extracelluluar domain, a five membrane-spanning segment and a short terminal cytoplasmic region. It is widely expressed on many cell types and often associated with beta 3 integrins. It has been reported that CD47 functions as a self marker. Red cells lacking CD47 were rapidly cleared from the bloodstream by splenic macrophages. By binding to SIRPα, CD47 controls hemostatic innate immune functions, such as phagocytosis and cell trafficking.

Protocols & Troubleshootings

Related Protocols Staining Cell Surface Targets for Flow Cytometry
Troubleshooting

Troubleshooting for Flow Cytometry

Alternative Formats

Product Name Sizes Price
AF/LE Purified Anti-Human/Mouse/Rat CD47 Antibody[MIAP410] 50μg, 500μg, 1mg Check More
Purified Anti-Human/Mouse/Rat CD47 Antibody[MIAP410] 25μg, 100μg Check More
Biotin Anti-Human/Mouse/Rat CD47 Antibody[MIAP410] 25μg, 100μg Check More
FITC Anti-Human/Mouse/Rat CD47 Antibody[MIAP410] 20Tests, 100Tests, 100Tests×2 Check More
PE Anti-Human/Mouse/Rat CD47 Antibody[MIAP410] 20Tests, 100Tests, 100Tests×2 Check More
APC Anti-Human/Mouse/Rat CD47 Antibody[MIAP410] 20Tests, 100Tests, 100Tests×2 Check More
PerCP Anti-Human/Mouse/Rat CD47 Antibody[MIAP410] 20Tests, 100Tests, 100Tests×2 Check More
PE/Cyanine5 Anti-Human/Mouse/Rat CD47 Antibody[MIAP410] 20Tests, 100Tests, 100Tests×2 Check More
PE/Cyanine5.5 Anti-Human/Mouse/Rat CD47 Antibody[MIAP410] 20Tests, 100Tests, 100Tests×2 Check More
PerCP/Cyanine5.5 Anti-Human/Mouse/Rat CD47 Antibody[MIAP410] 20Tests, 100Tests, 100Tests×2 Check More
Elab Fluor® 488 Anti-Human/Mouse/Rat CD47 Antibody[MIAP410] 20Tests, 100Tests, 100Tests×2 Check More
Elab Fluor® Violet 450 Anti-Human/Mouse/Rat CD47 Antibody[MIAP410] 20Tests, 100Tests, 100Tests×2 Check More
FITC Anti-Human/Mouse/Rat CD47 Antibody[MIAP410] 25μg, 100μg Check More
PE Anti-Human/Mouse/Rat CD47 Antibody[MIAP410] 25μg, 100μg Check More
APC Anti-Human/Mouse/Rat CD47 Antibody[MIAP410] 25μg, 100μg Check More
PerCP Anti-Human/Mouse/Rat CD47 Antibody[MIAP410] 25μg, 100μg Check More
PE/Cyanine5 Anti-Human/Mouse/Rat CD47 Antibody[MIAP410] 25μg, 100μg Check More
PE/Cyanine7 Anti-Human/Mouse/Rat CD47 Antibody[MIAP410] 25μg, 100μg Check More
PE/Cyanine5.5 Anti-Human/Mouse/Rat CD47 Antibody[MIAP410] 25μg, 100μg Check More
PerCP/Cyanine5.5 Anti-Human/Mouse/Rat CD47 Antibody[MIAP410] 25μg, 100μg Check More
Elab Fluor® 488 Anti-Human/Mouse/Rat CD47 Antibody[MIAP410] 25μg, 100μg Check More
Elab Fluor® Violet 450 Anti-Human/Mouse/Rat CD47 Antibody[MIAP410] 25μg, 100μg Check More

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