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PE/Cyanine7 Anti-Mouse CD103 Antibody[M290] E-AB-F1090H

Uniprot : Q60677

Cat.No.:E-AB-F1090H
Applications: FCM
Isotype: Rat IgG2a, κ
Host: Rat

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Antigen Details

Alternate Names	Integrin alpha-E,Itgae,Integrin alpha M290,CD103
Swissprot	Q60677
References	1. Kilshaw PJ and SJ. Murant. 1990. Eur. J. Immunol. 20:2201. 2. Karecla PI, et al. 1995. Eur. J. Immunol. 25:852. 3. LeFrancois L, et al. 1994. Eur. J. Immunol. 24:635. 4. Sung SS, et al. 2006. J. Immunol. 176:2161. 5. Johansson-Lindbom B, et al. 2005. J. Exp. Med. 202:1063. 6. Dujardin HC, et al. 2004. Proc. Natl. Acad. Sci. USA. 101:14473.

Product Details

Form	Liquid
Clone No.	M290(See Other Available Formats)
Host	Rat
Isotype	Rat IgG2a, κ
Isotype Control	PE/Cyanine7 Rat IgG2a, κ Isotype Control[2A3] [Product E-AB-F09832H]
Reactivity	Mouse
Application	FCM (See the Application Description)
Storage Buffer	Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use	Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping	Biological ice pack at 4℃
Stability & Storage	Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Conjugation	PE/Cyanine7
	PE/Cyanine7 is designed to be excited by the Blue (488 nm), Green (532 nm) and yellow-green (561 nm) lasers and detected using an optical filter centered near 775 nm (e.g., a 780/60 nm bandpass filter).

How to select the appropriate detection channel through the spectrogram?

Background

CD103 is a type I transmembrane glycoprotein known as αE integrin or Integrin αIEL chain. It belongs to the integrin family and is primarily found on intestinal intraepithelial lymphocytes (IEL). CD103 is also expressed on a subpopulation of lamina propria T cells, epithelial dendritic cells, lamina propria-derived dendritic cells, and a small subset of peripheral lymphocytes. T regulatory cells express high level of CD103. The CD103 expression on lymphocytes can be induced upon activation and TGF-β stimulation. In association with integrin β7, CD103 is expressed as αE/β7 heterodimer. Mature CD103 protein can be cleaved into 2 chains, a 150 kD (C-terminal) chain and a 25 kD (N-terminal) chain, which remain linked by disulfide bonds. CD103 binds to E-cadherin and mediates homing of lymphocytes to the intestinal epithelium.

Protocols & Troubleshootings

Related Protocols	Staining Cell Surface Targets for Flow Cytometry
Troubleshooting	Troubleshooting for Flow Cytometry

Alternative Formats

Product Name	Sizes	Price
AF/LE Purified Anti-Mouse CD103 Antibody[M290] E-AB-F10900	50μg, 500μg, 1mg	Check More
Purified Anti-Mouse CD103 Antibody[M290] E-AB-F1090A	25μg, 100μg	Check More
Biotin Anti-Mouse CD103 Antibody[M290] E-AB-F1090B	25μg, 100μg	Check More
FITC Anti-Mouse CD103 Antibody[M290] E-AB-F1090C	50Tests, 100Tests, 100Tests×2	Check More
PE Anti-Mouse CD103 Antibody[M290] E-AB-F1090D	50Tests, 100Tests, 100Tests×2	Check More
APC Anti-Mouse CD103 Antibody[M290] E-AB-F1090E	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5 Anti-Mouse CD103 Antibody[M290]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5.5 Anti-Mouse CD103 Antibody[M290] E-AB-F1090I	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 488 Anti-Mouse CD103 Antibody[M290]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 647 Anti-Mouse CD103 Antibody[M290]	50Tests, 100Tests, 100Tests×2	Check More
FITC Anti-Mouse CD103 Antibody[M290] E-AB-F1090UC	25μg, 100μg	Check More
PE Anti-Mouse CD103 Antibody[M290] E-AB-F1090UD	25μg, 100μg	Check More
APC Anti-Mouse CD103 Antibody[M290] E-AB-F1090UE	25μg, 100μg	Check More
PE/Cyanine5 Anti-Mouse CD103 Antibody[M290]	25μg, 100μg	Check More
PE/Cyanine7 Anti-Mouse CD103 Antibody[M290] E-AB-F1090UH	25μg, 100μg	Check More
PE/Cyanine5.5 Anti-Mouse CD103 Antibody[M290] E-AB-F1090UI	25μg, 100μg	Check More
Elab Fluor^® 488 Anti-Mouse CD103 Antibody[M290]	25μg, 100μg	Check More
Elab Fluor^® 647 Anti-Mouse CD103 Antibody[M290]	25μg, 100μg	Check More

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