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PE/Cyanine7 Anti-Mouse CD103 Antibody[M290] E-AB-F1090UH

Uniprot : Q60677
  • Cat.No.:E-AB-F1090UH

  • Applications: FCM
  • Isotype: Rat IgG2a, κ
  • Host: Rat

To Purchase E-AB-F1090UH

Size:
  • 25μg
  • 100μg
Price: $120
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Antigen Details

Alternate Names Integrin alpha-E,Itgae,Integrin alpha M290,CD103
Swissprot Q60677
References
1. Kilshaw PJ and SJ. Murant. 1990. Eur. J. Immunol. 20:2201.
2. Karecla PI, et al. 1995. Eur. J. Immunol. 25:852.
3. LeFrancois L, et al. 1994. Eur. J. Immunol. 24:635.
4. Sung SS, et al. 2006. J. Immunol. 176:2161.
5. Johansson-Lindbom B, et al. 2005. J. Exp. Med. 202:1063.
6. Dujardin HC, et al. 2004. Proc. Natl. Acad. Sci. USA. 101:14473.

Product Details

Form Liquid
Clone No. M290(See Other Available Formats)
Host Rat
Isotype Rat IgG2a, κ
Isotype Control PE/Cyanine7 Rat IgG2a, κ Isotype Control[2A3] [Product E-AB-F09833H]
Reactivity Mouse
Application FCM (See the Application Description)
Storage Buffer Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/106 cells in 100 μL volume].
Shipping Biological ice pack at 4℃
Stability & Storage Keep as concentrated solution.
Store at 2~8°C and protected from prolonged exposure to light. Do not freeze.
Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.
Conjugation PE/Cyanine7
PE/Cyanine7 is designed to be excited by the Blue (488 nm), Green (532 nm) and yellow-green (561 nm) lasers and detected using an optical filter centered near 775 nm (e.g., a 780/60 nm bandpass filter).


How to select the appropriate detection channel through the spectrogram?

Background

CD103 is a type I transmembrane glycoprotein known as αE integrin or Integrin αIEL chain. It belongs to the integrin family and is primarily found on intestinal intraepithelial lymphocytes (IEL). CD103 is also expressed on a subpopulation of lamina propria T cells, epithelial dendritic cells, lamina propria-derived dendritic cells, and a small subset of peripheral lymphocytes. T regulatory cells express high level of CD103. The CD103 expression on lymphocytes can be induced upon activation and TGF-β stimulation. In association with integrin β7, CD103 is expressed as αE/β7 heterodimer. Mature CD103 protein can be cleaved into 2 chains, a 150 kD (C-terminal) chain and a 25 kD (N-terminal) chain, which remain linked by disulfide bonds. CD103 binds to E-cadherin and mediates homing of lymphocytes to the intestinal epithelium.

Protocols & Troubleshootings

Related Protocols Staining Cell Surface Targets for Flow Cytometry
Troubleshooting

Troubleshooting for Flow Cytometry

Alternative Formats

Product Name Sizes Price
AF/LE Purified Anti-Mouse CD103 Antibody[M290] E-AB-F10900 50μg, 500μg, 1mg Check More
Purified Anti-Mouse CD103 Antibody[M290] E-AB-F1090A 25μg, 100μg Check More
Biotin Anti-Mouse CD103 Antibody[M290] E-AB-F1090B 25μg, 100μg Check More
FITC Anti-Mouse CD103 Antibody[M290] E-AB-F1090C 50Tests, 100Tests, 100Tests×2 Check More
PE Anti-Mouse CD103 Antibody[M290] E-AB-F1090D 50Tests, 100Tests, 100Tests×2 Check More
APC Anti-Mouse CD103 Antibody[M290] E-AB-F1090E 50Tests, 100Tests, 100Tests×2 Check More
PE/Cyanine5 Anti-Mouse CD103 Antibody[M290] 50Tests, 100Tests, 100Tests×2 Check More
PE/Cyanine7 Anti-Mouse CD103 Antibody[M290] E-AB-F1090H 50Tests, 100Tests, 100Tests×2 Check More
PE/Cyanine5.5 Anti-Mouse CD103 Antibody[M290] E-AB-F1090I 50Tests, 100Tests, 100Tests×2 Check More
Elab Fluor® 488 Anti-Mouse CD103 Antibody[M290] 50Tests, 100Tests, 100Tests×2 Check More
Elab Fluor® 647 Anti-Mouse CD103 Antibody[M290] 50Tests, 100Tests, 100Tests×2 Check More
FITC Anti-Mouse CD103 Antibody[M290] E-AB-F1090UC 25μg, 100μg Check More
PE Anti-Mouse CD103 Antibody[M290] E-AB-F1090UD 25μg, 100μg Check More
APC Anti-Mouse CD103 Antibody[M290] E-AB-F1090UE 25μg, 100μg Check More
PE/Cyanine5 Anti-Mouse CD103 Antibody[M290] 25μg, 100μg Check More
PE/Cyanine5.5 Anti-Mouse CD103 Antibody[M290] E-AB-F1090UI 25μg, 100μg Check More
Elab Fluor® 488 Anti-Mouse CD103 Antibody[M290] 25μg, 100μg Check More
Elab Fluor® 647 Anti-Mouse CD103 Antibody[M290] 25μg, 100μg Check More

Citations

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