Alternate Names	OX-2 membrane glycoprotein,OX-2, MRC
Swissprot	O54901
References	1. Hoek RM, et al. 2000. Science 290:1768. 2. Gorczynski R, et al. 2004. J. of Immunol. 172:7744. 3. Gorczynski L, et al. 1999. J. Immunol. 162:774. 4. Rosenblum MD, et al. 2004. Blood 103:2691. 5. Zhang S, et al. 2004. J. of Immunol. 173:6786. 6. Barclay AN, et al. 2002. Trends Immunol. 23:285.

Product Details

Form	Liquid
Clone No.	OX-90(See Other Available Formats)
Host	Rat
Isotype	Rat IgG2a, κ
Isotype Control	PE/Cyanine7 Rat IgG2a, κ Isotype Control[2A3] [Product E-AB-F09833H]
Reactivity	Mouse
Application	FCM (See the Application Description)
Storage Buffer	Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use	Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/10⁶ cells in 100 μL volume].
Shipping	Biological ice pack at 4℃
Stability & Storage	Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Conjugation	PE/Cyanine7
	PE/Cyanine7 is designed to be excited by the Blue (488 nm), Green (532 nm) and yellow-green (561 nm) lasers and detected using an optical filter centered near 775 nm (e.g., a 780/60 nm bandpass filter).

How to select the appropriate detection channel through the spectrogram?

Background

CD200 (OX-2 antigen) is a type-1 membrane glycoprotein containing two extracellular Ig-like domains. CD200 a highly conserved type I membrane glycoprotein that is expressed on a variety of cell types including thymocytes, some T cells, endothelial and follicular dendritc cells, B cells, and brain tissue (neurons); but not on NK cells, granulocytes, monocytes, or macrophages. CD200 costimulates T cell proliferation. It may regulate myeloid cell activity in a variety of tissues. CD200 is the ligand for CD200 receptor (CD200R). The CD200 Receptor is restricted to myeloid cells, and it is believed that its engagement with CD200 results in inhibition and/or downregulation of myeloid cell activity. Blocking of CD200/CD200R interactions decreases myeloid cell inhibitory thresholds which results in enhanced immune activation.

Protocols & Troubleshootings

Related Protocols	Staining Cell Surface Targets for Flow Cytometry
Troubleshooting	Troubleshooting for Flow Cytometry

Alternative Formats

Product Name	Sizes	Price
APC Anti-Mouse CD200/OX2 Antibody[OX-90]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine7 Anti-Mouse CD200/OX2 Antibody[OX-90]	50Tests, 100Tests, 100Tests×2	Check More
APC Anti-Mouse CD200/OX2 Antibody[OX-90]	25μg, 100μg	Check More

Citations

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