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PE/Cyanine7 Anti-Mouse CD272/BTLA Antibody[PK18.6]

Uniprot : Q7TSA3
  • Cat.No.:E-AB-F1024UH

  • Applications: FCM
  • Isotype: Rat IgG1, κ
  • Host: Rat

To Purchase E-AB-F1024UH

Size:
  • 25μg
  • 100μg
Price: $130
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Antigen Details

Alternate Names B- and T-lymphocyte attenuator,Btla,B- and T-lymphocyte-associated protein,CD272,Btla
Swissprot Q7TSA3
References
1. Liu X, et al. 2009. J. Immunol. 182:4516.
2. Miller ML, et al. 2009. J. Immunol. 183:32.
3. Sun Y, et al. 2009. J. Immunol. 183:1946.
4. Vendel AC, et al. 2009. J. Immunol. 182:1509.
5. Watanabe N, et al. 2003. Nat. Immunol. 4:670.

Product Details

Form Liquid
Clone No. PK18.6(See Other Available Formats)
Host Rat
Isotype Rat IgG1, κ
Isotype Control PE/Cyanine7 Rat IgG1, κ Isotype Control[HRPN] [Product E-AB-F09823H]
Reactivity Mouse
Application FCM (See the Application Description)
Storage Buffer Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/106 cells in 100 μL volume].
Shipping Biological ice pack at 4℃
Stability & Storage Keep as concentrated solution.
Store at 2~8°C and protected from prolonged exposure to light. Do not freeze.
Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.
Conjugation PE/Cyanine7
PE/Cyanine7 is designed to be excited by the Blue (488 nm), Green (532 nm) and yellow-green (561 nm) lasers and detected using an optical filter centered near 775 nm (e.g., a 780/60 nm bandpass filter).


How to select the appropriate detection channel through the spectrogram?

Background

CD272, also known as B and T lymphocyte attenuator (BTLA), is an Ig superfamily co-inhitory receptor with structural similarity to programmed cell death 1 (PD-1) and CTLA-4. BTLA is expressed on B cells, T cells, macrophages, dendritic cells, NKT cells, and NK cells. Engagement of BTLA by its ligand herpes virus entry mediator (HVEM) is critical for negatively regulating immune response. The absence of BTLA with HVEM inhibitory interactions leads to increased experimental autoimmune encephalomyelitis severity, enhanced rejection of partially mismatched allografts, an increased CD8+ memory T cell population, increased severity of colitis, and reduced effectiveness of T regulatory cells. BTLA plays an important role in the induction of peripheral tolerance of both CD4+ and CD8+ T cells in vivo. Tolerant T cells have significantly higher expression of BTLA compared with effectors and naïve T cells. BTLA may cooperate with CTLA-4 and PD-1 to control T cell tolerance and autoimmunity. It was reported that BTLA may regulate T cell function by binding to B7-H4, but further studies are needed to confirm. The existence of three distinct BTLA alleles has been reported.

Protocols & Troubleshootings

Related Protocols Staining Cell Surface Targets for Flow Cytometry
Troubleshooting

Troubleshooting for Flow Cytometry

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