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PE/Cyanine7 Anti-Mouse CD69 Antibody[H1.2F3]

  • Cat.No.:E-AB-F1187H

  • Applications: FCM
  • Isotype: Armenian Hamster IgG
  • Host: Armenian Hamster

To Purchase E-AB-F1187H

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  • 50Tests
  • 100Tests
  • 100Tests×2
Price: $115
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Antigen Details

Alternate Names AIM,CLEC2C,GP32/28,MLR-3,VEA,EA1
Swissprot P37217
Gene ID 12515
References
1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Testi R, et al. 1994. Immunol. Today 15:479.
3. Moretta A, et al. 1991. J. Exp. Med. 174:1393.
4. Yokoyama WM, et al. 1988. J. Immunol. 141:369.

Product Details

Form Liquid
Clone No. H1.2F3(See Other Available Formats)
Host Armenian Hamster
Isotype Armenian Hamster IgG
Isotype Control PE/Cyanine7 Armenian Hamster IgG Isotype Control[PIP] [Product E-AB-F09852H]
Reactivity Mouse
Application FCM (See the Application Description)
Storage Buffer Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping Biological ice pack at 4℃
Stability & Storage Keep as concentrated solution.
Store at 2~8°C and protected from prolonged exposure to light. Do not freeze.
Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.
Conjugation PE/Cyanine7
PE/Cyanine7 is designed to be excited by the Blue (488 nm), Green (532 nm) and yellow-green (561 nm) lasers and detected using an optical filter centered near 775 nm (e.g., a 780/60 nm bandpass filter).


How to select the appropriate detection channel through the spectrogram?

Background

CD69 is a 60 kD type II membrane protein composed of a 27/33 kD disulfide-linked homodimer, also known as Very Early Activation Antigen (VEA), AIM, EA1, MLR3, and gp34/28. It is expressed on a subset of thymocytes and platelets. CD69 is rapidly induced on activated T and B cells, neutrophils, and NK cells. It is a C-type lectin, closely related to the NKR-P1 and Ly-49 NK cell activation molecules. CD69 is involved in the early events of cell activation and thymocyte positive selection.

Protocols & Troubleshootings

Related Protocols Staining Cell Surface Targets for Flow Cytometry
Troubleshooting

Troubleshooting for Flow Cytometry

Citations

  1. Frontiers in Immunology (2022) IF: 7.561
    Targeting Glutamine Metabolism Ameliorates Autoimmune Hepatitis via Inhibiting T Cell Activation and Differentiation.

    DOI: 10.3389/fimmu.2022.880262

    Sample: splenocytes

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