Alternate Names	Ly6a,Ly6,Ly-6A.2/Ly-6E.1,SCA-1,TAP
Swissprot	P05533
References	1. Rock KL, et al. 1989. Immunol. Rev. 111:195. 2. Morrison SJ, et al. 1994. Immunity 1:661. 3. Spangrude GJ, et al. 1988. J. Immunol. 141:3697. 4. Malek T, et al. 1986. J. Exp. Med. 164:709.

Product Details

Form	Liquid
Clone No.	D7(See Other Available Formats)
Host	Rat
Isotype	Rat IgG2a, κ
Isotype Control	PE/Cyanine7 Rat IgG2a, κ Isotype Control[2A3] [Product E-AB-F09833H]
Reactivity	Mouse
Application	FCM (See the Application Description)
Storage Buffer	Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use	Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/10⁶ cells in 100 μL volume].
Shipping	Biological ice pack at 4℃
Stability & Storage	Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Conjugation	PE/Cyanine7
	PE/Cyanine7 is designed to be excited by the Blue (488 nm), Green (532 nm) and yellow-green (561 nm) lasers and detected using an optical filter centered near 775 nm (e.g., a 780/60 nm bandpass filter).

How to select the appropriate detection channel through the spectrogram?

Background

Ly-6A/E, also known as Sca-1, is an 18 kD member of the Ly-6 multigene family. Ly6A/E is a glycosylphosphatidylinositol (GPI)-linked protein expressed on hematopoietic stem cells. In mice expressing the Ly-6.2 haplotype (e.g., AKR, C57BL, C57BR, DBA/2, SJL, SWR, and 129), Ly-6A/E is also expressed on peripheral B lymphocytes and thymic and peripheral T lymphocytes. Strains expressing the Ly-6.1 haplotype (e.g., BALB/c, CBA, C3H/He, DBA/1, and NZB) have low Ly-6A/E expression on resting peripheral lymphocytes. The expression of Ly-6A/E on lymphocytes is upregulated upon activation from both Ly6.1 and Ly6.2 haplotype mice. Ly-6A/E is thought to be involved in the regulation of both T and B cell responses.

Protocols & Troubleshootings

Related Protocols	Staining Cell Surface Targets for Flow Cytometry
Troubleshooting	Troubleshooting for Flow Cytometry

Alternative Formats

Product Name	Sizes	Price
FITC Anti-Mouse Ly6A/E(Sca-1) Antibody[D7]	50Tests, 100Tests, 100Tests×2	Check More
PE Anti-Mouse Ly6A/E(Sca-1) Antibody[D7]	50Tests, 100Tests, 100Tests×2	Check More
APC Anti-Mouse Ly6A/E(Sca-1) Antibody[D7]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5 Anti-Mouse Ly6A/E(Sca-1) Antibody[D7]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine7 Anti-Mouse Ly6A/E(Sca-1) Antibody[D7]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5.5 Anti-Mouse Ly6A/E(Sca-1) Antibody[D7]	50Tests, 100Tests, 100Tests×2	Check More
PerCP/Cyanine5.5 Anti-Mouse Ly6A/E(Sca-1) Antibody[D7]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 488 Anti-Mouse Ly6A/E(Sca-1) Antibody[D7]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 647 Anti-Mouse Ly6A/E(Sca-1) Antibody[D7]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® Violet 450 Anti-Mouse Ly6A/E(Sca-1) Antibody[D7]	50Tests, 100Tests, 100Tests×2	Check More
FITC Anti-Mouse Ly6A/E(Sca-1) Antibody[D7]	25μg, 100μg	Check More
PE Anti-Mouse Ly6A/E(Sca-1) Antibody[D7]	25μg, 100μg	Check More
APC Anti-Mouse Ly6A/E(Sca-1) Antibody[D7]	25μg, 100μg	Check More
PE/Cyanine5 Anti-Mouse Ly6A/E(Sca-1) Antibody[D7]	25μg, 100μg	Check More
PE/Cyanine5.5 Anti-Mouse Ly6A/E(Sca-1) Antibody[D7]	25μg, 100μg	Check More
PerCP/Cyanine5.5 Anti-Mouse Ly6A/E(Sca-1) Antibody[D7]	25μg, 100μg	Check More
Elab Fluor^® 488 Anti-Mouse Ly6A/E(Sca-1) Antibody[D7]	25μg, 100μg	Check More
Elab Fluor^® 647 Anti-Mouse Ly6A/E(Sca-1) Antibody[D7]	25μg, 100μg	Check More
Elab Fluor^® Violet 450 Anti-Mouse Ly6A/E(Sca-1) Antibody[D7]	25μg, 100μg	Check More

Citations

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