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PE/Cyanine7 Anti-Rat CD44H Antibody[OX-49]

Uniprot : P26051
  • Cat.No.:E-AB-F1225UH

  • Applications: FCM
  • Isotype: Mouse IgG2a, κ
  • Host: Mouse

To Purchase E-AB-F1225UH

Size:
  • 25μg
  • 100μg
Price: $90
Qty:

Antigen Details

Alternate Names Pgp-1, H-CAM, CD44s,CD44H
Swissprot P26051
Gene ID 12505
References
1. Arch R, et al. 1992. Science 257 (5070):682
2. Mitnacht R, et al. 1995. Eur. J. Immunol. 25(2):328
3. Zheng Z, et al. 1995. J Cell Bio. 130(2):485
4. Westermann J, et al. 1994. Eur. J. Immunol. 24(10):2312

Product Details

Form Liquid
Clone No. OX-49(See Other Available Formats)
Host Mouse
Isotype Mouse IgG2a, κ
Isotype Control PE/Cyanine7 Mouse IgG2a, κ Isotype Control[C1.18.4] [Product E-AB-F09803H]
Reactivity Rat
Application FCM (See the Application Description)
Storage Buffer Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/106 cells in 100 μL volume].
Shipping Biological ice pack at 4℃
Stability & Storage Keep as concentrated solution.
Store at 2~8°C and protected from prolonged exposure to light. Do not freeze.
Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.
Conjugation PE/Cyanine7
PE/Cyanine7 is designed to be excited by the Blue (488 nm), Green (532 nm) and yellow-green (561 nm) lasers and detected using an optical filter centered near 775 nm (e.g., a 780/60 nm bandpass filter).


How to select the appropriate detection channel through the spectrogram?

Background

CD44 is an 80-95 kD cell surface glycoprotein. It is expressed on all leukocytes, endothelial cells, hepatocytes, and mesenchymal cells. It is up-regulated when T cells and B cells are activated. It was reported that CD44 is a valuable marker for memory T cells. CD44 is an adhesion molecule involved in leukocyte adhesion and homing to lymphoid organs. The OX-49 antibody reacts with CD44H (known as CD44s) expressed on most leukocytes, except for a subset of B lymphocytes. The epitope recognized by OX-49 antibody has been mapped to a region on both the standard, CD44s, and the splice variant, CD44v, isoforms of CD44. However it was reported that OX-49 antibody cannot detect the CD44V isoform, possibly due to conformational changes in the epitope.

Protocols & Troubleshootings

Related Protocols Staining Cell Surface Targets for Flow Cytometry
Troubleshooting

Troubleshooting for Flow Cytometry

Citations

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