Alternate Names	Bp35,Leukocyte surface antigen Leu-16,MS4A1,B-lymphocyte surface antigen B1
Swissprot	P11836
References	1. Hultin L, et al. 1993. Cytometry 14:196. 2. Tedder T, et al. 1994. Immunol. Today 15:450.

Product Details

Form	Liquid
Clone No.	2H7(See Other Available Formats)
Host	Mouse
Isotype	Mouse IgG2b, κ
Isotype Control	PerCP/Cyanine5.5 Mouse IgG2b, κ Isotype Control[MPC-11] [Product E-AB-F09812J]
Reactivity	Human
Application	FCM (See the Application Description)
Storage Buffer	Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use	Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping	Biological ice pack at 4℃
Stability & Storage	Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Conjugation	PerCP/Cyanine5.5
	PerCP/Cyanine5.5 is designed to be excited by the blue laser (488 nm) and detected using an optical filter centered near 675 nm (e.g., a 690/50 nm bandpass filter).

How to select the appropriate detection channel through the spectrogram?

Background

CD20 is a 33-37 kD, four transmembrane spanning protein, also known as B1 and Bp35. CD20 is expressed on pre-B-cells, resting and activated B cells (not plasma cells), some follicular dendritic cells, and at low levels on a T cell subset. CD20 is heavily phosphorylated on activated B cells and malignant B cells. Homo-oligomeric complexes of CD20 are thought to form Ca2+ conductive ion channels in the plasma membrane of B cells. The CD20 molecule is involved in B-cell activation and is associated with various Src family kinases (Lyn, Lck, Fyn). It exists in a complex with MHC class I and II, CD53, CD81, and CD82.

Protocols & Troubleshootings

Related Protocols	Staining Cell Surface Targets for Flow Cytometry
Troubleshooting	Troubleshooting for Flow Cytometry

Alternative Formats

Product Name	Sizes	Price
AF/LE Purified Anti-Human CD20 Antibody[2H7]	50μg, 500μg, 1mg	Check More
Purified Anti-Human CD20 Antibody[2H7]	25μg, 100μg	Check More
FITC Anti-Human CD20 Antibody[2H7]	20Tests, 100Tests, 100Tests×2	Check More
PE Anti-Human CD20 Antibody[2H7]	20Tests, 100Tests, 100Tests×2	Check More
APC Anti-Human CD20 Antibody[2H7]	20Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5 Anti-Human CD20 Antibody[2H7]	20Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine7 Anti-Human CD20 Antibody[2H7]	20Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5.5 Anti-Human CD20 Antibody[2H7]	20Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 488 Anti-Human CD20 Antibody[2H7]	20Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 647 Anti-Human CD20 Antibody[2H7]	20Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® Violet 450 Anti-Human CD20 Antibody[2H7]	20Tests, 100Tests, 100Tests×2	Check More

Citations

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