Current Position:Home>>Antibodies>>Flow Cytometry Antibodies>> PerCP/Cyanine5.5 Anti-Human CD28 Antibody[CD28.2] E-AB-F1195J

PerCP/Cyanine5.5 Anti-Human CD28 Antibody[CD28.2] E-AB-F1195J

Uniprot : P10747

Cat.No.:E-AB-F1195J
Reactivity: Human

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Antigen Details

Alternate Names	PAS2,MNS,HGpMiX,GYPA,GPSAT
Swissprot	P10747
References	1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. 2. June CH, et al. 1994. Immunol. Today 15:321. 3. Linskey PS, et al. 1993. Annu. Rev. Immunol. 11:191.

Product Details

Form	Liquid
Clone No.	CD28.2(See Other Available Formats)
Host	Mouse
Isotype	Mouse IgG1, κ
Isotype Control	PerCP/Cyanine5.5 Mouse IgG1, κ Isotype Control[MOPC-21] [Product E-AB-F09792J]
Reactivity	Human
Application	FCM (See the Application Description)
Storage Buffer	PBS with 0.05% Proclin300, 1% BSA
Recommended Use	Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The amount of the reagent is suggested to be used 5 μL of antibody per test (million cells in 100 μL staining volume or per 100 μL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping	Biological ice pack at 4℃
Stability & Storage	Keep as concentrated solution. Store at 2~8℃ and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Conjugation	PerCP/Cyanine5.5

Background

CD28 is a 44 kD disulfide-linked homodimeric type I glycoprotein. It is a member of the immunoglobulin superfamily and is also known as T44 or Tp44. CD28 is expressed on most T lineage cells, NK cell subsets, and plasma cells. CD28 binds both CD80 and CD86 using a highly conserved motif MYPPY in the CDR3-like loop. CD28 is considered a major co-stimulatory molecule, inducing T lymphocyte activation and IL-2 synthesis, and preventing cell death. In vitro studies indicate that ligation of CD28 on T cells by CD80 and CD86 on antigen presenting cells provides a costimulatory signal required for T cell activation and proliferation.

Protocols & Troubleshootings

Related Protocols	Staining Cell Surface Targets for Flow Cytometry
Troubleshooting	Troubleshooting for Flow Cytometry

Alternative Formats

Cat.No.	Product Name	Sizes
E-AB-F11950	AF/LE Purified Anti-Human CD28 Antibody[CD28.2] E-AB-F11950	50μg, 500μg, 1mg
E-AB-F1195A	Purified Anti-Human CD28 Antibody[CD28.2] E-AB-F1195A	25μg, 100μg
E-AB-F1195C	FITC Anti-Human CD28 Antibody[CD28.2] E-AB-F1195C	20Tests, 50Tests, 100Tests, 200Tests
E-AB-F1195D	PE Anti-Human CD28 Antibody[CD28.2] E-AB-F1195D	20Tests, 50Tests, 100Tests, 200Tests
E-AB-F1195E	APC Anti-Human CD28 Antibody[CD28.2] E-AB-F1195E	20Tests, 50Tests, 100Tests, 200Tests
E-AB-F1195G	PE/Cyanine5 Anti-Human CD28 Antibody[CD28.2] E-AB-F1195G	20Tests, 50Tests, 100Tests, 200Tests
E-AB-F1195H	PE/Cyanine7 Anti-Human CD28 Antibody[CD28.2] E-AB-F1195H	20Tests, 50Tests, 100Tests, 200Tests
E-AB-F1195I
E-AB-F1195J	PerCP/Cyanine5.5 Anti-Human CD28 Antibody[CD28.2] E-AB-F1195J	20Tests, 50Tests, 100Tests, 200Tests
E-AB-F1195L	AF488 Anti-Human CD28 Antibody[CD28.2] E-AB-F1195L	20Tests, 50Tests, 100Tests, 200Tests
E-AB-F1195M	AF647 Anti-Human CD28 Antibody[CD28.2] E-AB-F1195M	20Tests, 50Tests, 100Tests, 200Tests

Citations

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